使用热水而非二甲苯对福尔马林固定石蜡包埋组织块进行脱石蜡处理。

Deparaffinization of formalin-fixed paraffin-embedded tissue blocks using hot water instead of xylene.

作者信息

Kalantari Narges, Bayani Masomeh, Ghaffari Taraneh

机构信息

Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran.

出版信息

Anal Biochem. 2016 Aug 15;507:71-3. doi: 10.1016/j.ab.2016.05.015. Epub 2016 Jun 7.

DOI:10.1016/j.ab.2016.05.015

PMID:27287960

Abstract

This study aimed to deparaffinize formalin-fixed paraffin-embedded (FFPE) tissues using hot water instead of xylene and measuring the quantity and quality of the extracted DNA from the respective tissues. To deparaffinize the tissue sections with hot water, small sections were exposed to 90 °C distilled sterile water. After 25 FFPE tissue samples were deparaffinized with the hot water method, DNA was then extracted. The mean of optical density and the ratio of absorbance of the DNA solution were 220.01 ± 36.1 ng/μl and 1.65 ± 0.1, respectively. Polymerase chain reaction (PCR) analysis of the toll-like receptor 4(TLR4) gene showed that the method can be used as a tool for different applications.

摘要

本研究旨在使用热水而非二甲苯对福尔马林固定石蜡包埋（FFPE）组织进行脱石蜡处理，并测量从相应组织中提取的DNA的数量和质量。为了用热水对组织切片进行脱石蜡处理，将小切片置于90°C无菌蒸馏水中。在用热水法对25个FFPE组织样本进行脱石蜡处理后，接着提取DNA。DNA溶液的光密度平均值和吸光度比值分别为220.01±36.1 ng/μl和1.65±0.1。Toll样受体4（TLR4）基因的聚合酶链反应（PCR）分析表明，该方法可作为一种用于不同应用的工具。