Plácido Alexandra, de Oliveira Farias Emanuel Airton, Marani Mariela M, Gomes Vasconcelos Andreanne, Leite José R S A, Delerue-Matos Cristina
REQUIMTE/LAQV, Instituto Superior de Engenharia do Porto, ISEP, Instituto Politécnico do Porto, Porto, Portugal.
Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Velloso, CMRV, Universidade Federal do Piauí, UFPI, Parnaíba, PI, Brasil.
Data Brief. 2016 May 21;8:114-9. doi: 10.1016/j.dib.2016.05.031. eCollection 2016 Sep.
The peptide PcL342-354C was obtained from the Cry1Ab16 toxin present in Bacillus thuringiensis ("Computational Modeling Deduced Three Dimensional Structure of Cry1Ab16 Toxin from B. thuringiensis AC11" (Kashyap, 2012) [1]). In this data article, we report the synthesis and characterization of the PcL342-354C peptide by MALDI-TOF/TOF mass spectrometry. In addition, the preparation of layer-by-layer films is shown based on interspersion of this peptide with both polyethylenimine (PEI) and poly(sodium 4-styrenesulfonate) (PSS), self-assembled on ITO (indium tin oxide) electrodes. The morphology of the ITO/PEI/PSS/PcL342-354C film was analyzed using atomic force microscopy (AFM). We also evaluated the effect of the number of bilayers in ITO/PEI/(PSS/PcL342-354C) n on the morphology of the film using AFM amplitude images. Further details about this study were published elsewhere, "Layer-by-layer films containing peptides of the Cry1Ab16 toxin from B. thuringiensis for potential biotechnological applications," (Plácido et al., 2016) [2].
肽段PcL342 - 354C是从苏云金芽孢杆菌中存在的Cry1Ab16毒素获得的（“Computational Modeling Deduced Three Dimensional Structure of Cry1Ab16 Toxin from B. thuringiensis AC11”（Kashyap，2012年）[1]）。在本数据文章中，我们报告了通过基质辅助激光解吸电离飞行时间/串联飞行时间质谱对PcL342 - 354C肽段进行的合成与表征。此外，展示了基于该肽段与聚乙烯亚胺（PEI）和聚（4 - 苯乙烯磺酸钠）（PSS）的交替穿插，在氧化铟锡（ITO）电极上自组装制备逐层薄膜的过程。使用原子力显微镜（AFM）分析了ITO/PEI/PSS/PcL342 - 354C薄膜的形态。我们还使用AFM振幅图像评估了ITO/PEI/(PSS/PcL342 - 354C)n中双层数对薄膜形态的影响。关于这项研究的更多详细信息发表在其他地方，“Layer - by - layer films containing peptides of the Cry1Ab16 toxin from B. thuringiensis for potential biotechnological applications”（Plácido等人，2016年）[2]。
