Gunsé Benet, Poschenrieder Charlotte, Rankl Simone, Schröeder Peter, Rodrigo-Moreno Ana, Barceló Juan
Lab. Fisiología Vegetal, Facultad Biociencias, Universidad Autónoma de Barcelona, E-08193 Bellaterra, Spain.
Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH), Ingolstädter Landstr. 1, 85764 Neuherberg, Germany.
MethodsX. 2016 May 25;3:436-51. doi: 10.1016/j.mex.2016.05.007. eCollection 2016.
In this study we present a highly versatile and easily configurable system for measuring plant electrophysiological parameters and ionic flow rates, connected to a computer-controlled highly accurate positioning device. The modular software used allows easy customizable configurations for the measurement of electrophysiological parameters. Both the operational tests and the experiments already performed have been fully successful and rendered a low noise and highly stable signal. Assembly, programming and configuration examples are discussed. The system is a powerful technique that not only gives precise measuring of plant electrophysiological status, but also allows easy development of ad hoc configurations that are not constrained to plant studies. •We developed a highly modular system for electrophysiology measurements that can be used either in organs or cells and performs either steady or dynamic intra- and extracellular measurements that takes advantage of the easiness of visual object-oriented programming.•High precision accuracy in data acquisition under electrical noisy environments that allows it to run even in a laboratory close to electrical equipment that produce electrical noise.•The system makes an improvement of the currently used systems for monitoring and controlling high precision measurements and micromanipulation systems providing an open and customizable environment for multiple experimental needs.
在本研究中,我们展示了一种高度通用且易于配置的系统,用于测量植物电生理参数和离子流速,该系统连接到计算机控制的高精度定位设备。所使用的模块化软件允许对电生理参数测量进行轻松的可定制配置。已进行的操作测试和实验均取得了圆满成功,并产生了低噪声和高度稳定的信号。文中讨论了组装、编程和配置示例。该系统是一项强大的技术,不仅能精确测量植物的电生理状态,还能轻松开发不限于植物研究的特殊配置。•我们开发了一种高度模块化的电生理测量系统,可用于器官或细胞,进行稳态或动态的细胞内和细胞外测量,利用了可视化面向对象编程的简便性。•在电噪声环境下具有高精度的数据采集能力,使其即使在靠近产生电噪声的电气设备的实验室中也能运行。•该系统改进了目前用于监测和控制高精度测量及微操纵系统的系统,为多种实验需求提供了一个开放且可定制的环境。