Leewis Mary-Cathrine, Uhlik Ondrej, Fraraccio Serena, McFarlin Kelly, Kottara Anastasia, Glover Catherine, Macek Tomas, Leigh Mary Beth
Institute of Arctic Biology, University of Alaska Fairbanks, Fairbanks AK, USA.
Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology Prague, Czech Republic.
Front Microbiol. 2016 Jun 2;7:837. doi: 10.3389/fmicb.2016.00837. eCollection 2016.
Despite decades of research there is limited understanding of how vegetation impacts the ability of microbial communities to process organic contaminants in soil. Using a combination of traditional and molecular assays, we examined how phytoremediation with willow and/or fertilization affected the microbial community present and active in the transformation of diesel contaminants. In a pot study, willow had a significant role in structuring the total bacterial community and resulted in significant decreases in diesel range organics (DRO). However, stable isotope probing (SIP) indicated that fertilizer drove the differences seen in community structure and function. Finally, analysis of the total variance in both pot and SIP experiments indicated an interactive effect between willow and fertilizer on the bacterial communities. This study clearly demonstrates that a willow native to Alaska accelerates DRO degradation, and together with fertilizer, increases aromatic degradation by shifting microbial community structure and the identity of active naphthalene degraders.
尽管经过了数十年的研究，但对于植被如何影响微生物群落处理土壤中有机污染物的能力，人们的了解仍然有限。我们结合传统检测方法和分子检测方法，研究了柳树植物修复和/或施肥如何影响参与柴油污染物转化的微生物群落的存在和活性。在盆栽试验中，柳树在构建总细菌群落方面发挥了重要作用，并导致柴油范围有机物（DRO）显著减少。然而，稳定同位素探测（SIP）表明，肥料导致了群落结构和功能上的差异。最后，盆栽试验和SIP试验的总方差分析表明，柳树和肥料对细菌群落有交互作用。这项研究清楚地表明，阿拉斯加本土的柳树能加速DRO的降解，并且与肥料一起，通过改变微生物群落结构和活性萘降解菌的种类来增加芳烃的降解。
