单细胞分析：进展与未来展望。
Single-cell analysis: Advances and future perspectives.
作者信息
Hodzic Emir
机构信息
University of California at Davis, School of Veterinary Medicine.
出版信息
Bosn J Basic Med Sci. 2016 Nov 10;16(4):313-314. doi: 10.17305/bjbms.2016.1371.
Abstract
The last several years have seen rapid development of technologies and methods that permit a detailed analysis of the genome and transcriptome of a single cell. Recent evidence from studies of single cells reveals that each cell type has a distinct lineage and function. The lineage and stage of development of each cell determine how they respond to each other and the environment. Experimental approaches that utilize single-cell analysis are effective means to understand how cell networks work in concert to coordinate a response at the population level; recent progress in single-cell analysis is offering a glimpse at the future.
摘要
在过去几年中，能够对单个细胞的基因组和转录组进行详细分析的技术和方法得到了迅速发展。来自单细胞研究的最新证据表明，每种细胞类型都有独特的谱系和功能。每个细胞的谱系和发育阶段决定了它们如何相互作用以及对环境的反应。利用单细胞分析的实验方法是了解细胞网络如何协同工作以在群体水平上协调反应的有效手段；单细胞分析的最新进展让我们看到了未来的曙光。
相似文献
1
Single-cell analysis: Advances and future perspectives.单细胞分析：进展与未来展望。
Bosn J Basic Med Sci. 2016 Nov 10;16(4):313-314. doi: 10.17305/bjbms.2016.1371.
2
Single cell genomics: advances and future perspectives.单细胞基因组学：进展与未来展望。
PLoS Genet. 2014 Jan 30;10(1):e1004126. doi: 10.1371/journal.pgen.1004126. eCollection 2014 Jan.
3
Primed and ready: understanding lineage commitment through single cell analysis.做好准备：通过单细胞分析了解谱系决定。
Trends Cell Biol. 2015 Aug;25(8):459-67. doi: 10.1016/j.tcb.2015.04.004. Epub 2015 May 22.
4
Single-cell sequencing technologies: current and future.单细胞测序技术：现状与未来
J Genet Genomics. 2014 Oct 20;41(10):513-28. doi: 10.1016/j.jgg.2014.09.005. Epub 2014 Oct 18.
5
[Recent progress in single-cell RNA-Seq analysis].[单细胞RNA测序分析的最新进展]
Yi Chuan. 2014 Nov;36(11):1069-76. doi: 10.3724/SP.J.1005.2014.1069.
6
Single-cell sequencing in stem cell biology.干细胞生物学中的单细胞测序
Genome Biol. 2016 Apr 15;17:71. doi: 10.1186/s13059-016-0941-0.
7
Quantitative single-cell approaches to stem cell research.定量单细胞方法在干细胞研究中的应用。
Cell Stem Cell. 2014 Nov 6;15(5):546-58. doi: 10.1016/j.stem.2014.10.015.
8
Single-Cell RNA-Seq of Bone Marrow-Derived Mesenchymal Stem Cells Reveals Unique Profiles of Lineage Priming.骨髓间充质干细胞的单细胞RNA测序揭示了谱系启动的独特特征。
PLoS One. 2015 Sep 9;10(9):e0136199. doi: 10.1371/journal.pone.0136199. eCollection 2015.
9
Studying hematopoiesis using single-cell technologies.使用单细胞技术研究造血。
J Hematol Oncol. 2017 Jan 21;10(1):27. doi: 10.1186/s13045-017-0401-7.
10
Single cell sequencing approaches for complex biological systems.用于复杂生物系统的单细胞测序方法。
Curr Opin Genet Dev. 2014 Jun;26:59-65. doi: 10.1016/j.gde.2014.06.004. Epub 2014 Jul 10.
引用本文的文献
1
Generative artificial intelligence, integrative bioinformatics, and single-cell analysis reveal Alzheimer's genetic and immune landscape.生成式人工智能、整合生物信息学和单细胞分析揭示了阿尔茨海默病的遗传和免疫格局。
Mol Ther Nucleic Acids. 2025 Apr 24;36(2):102546. doi: 10.1016/j.omtn.2025.102546. eCollection 2025 Jun 10.
2
Dielectrophoretic capture and electrochemical enzyme-linked immunosorbent assay of single melanoma cells at an array of interlocked spiral bipolar electrodes.在互锁螺旋双极电极阵列上对单个黑色素瘤细胞进行介电泳捕获和电化学酶联免疫吸附测定。
ChemElectroChem. 2024 Aug 1;11(15). doi: 10.1002/celc.202400182. Epub 2024 Jul 4.
3
Stochastic journeys of cell progenies through compartments and the role of self-renewal, symmetric and asymmetric division.细胞后代在隔室中的随机旅程和自我更新、对称和不对称分裂的作用。
Sci Rep. 2024 Jul 15;14(1):16287. doi: 10.1038/s41598-024-63500-2.
4
Optical diffraction tomography for assessing single cell models in angular light scattering.用于评估角向光散射中单细胞模型的光学衍射层析成像技术。
Biomed Opt Express. 2024 Jan 24;15(2):973-990. doi: 10.1364/BOE.512149. eCollection 2024 Feb 1.
5
Digital PCR for Single-Cell Analysis.数字 PCR 用于单细胞分析。
Biosensors (Basel). 2024 Jan 24;14(2):64. doi: 10.3390/bios14020064.
6
Preclinical Models of Cardiac Disease: A Comprehensive Overview for Clinical Scientists.心脏疾病的临床前模型：临床科学家的综合概述。
Cardiovasc Eng Technol. 2024 Apr;15(2):232-249. doi: 10.1007/s13239-023-00707-w. Epub 2024 Jan 16.
7
Droplets microfluidics platform-A tool for single cell research.微滴微流控平台——一种用于单细胞研究的工具。
Front Bioeng Biotechnol. 2023 Apr 19;11:1121870. doi: 10.3389/fbioe.2023.1121870. eCollection 2023.
8
Contribution of Retrotransposons to the Pathogenesis of Type 1 Diabetes and Challenges in Analysis Methods.逆转录转座子在 1 型糖尿病发病机制中的作用及分析方法中的挑战。
Int J Mol Sci. 2023 Feb 4;24(4):3104. doi: 10.3390/ijms24043104.
9
Inferencing Bulk Tumor and Single-Cell Multi-Omics Regulatory Networks for Discovery of Biomarkers and Therapeutic Targets.推断肿瘤组织和单细胞多组学调控网络，以发现生物标志物和治疗靶点。
Cells. 2022 Dec 26;12(1):101. doi: 10.3390/cells12010101.
10
Microfluidic chips: recent advances, critical strategies in design, applications and future perspectives.微流控芯片：最新进展、设计关键策略、应用及未来展望
Microfluid Nanofluidics. 2021;25(12):99. doi: 10.1007/s10404-021-02502-2. Epub 2021 Oct 26.
本文引用的文献
1
Digital Quantification of Proteins and mRNA in Single Mammalian Cells.单细胞内蛋白质和 mRNA 的数字量化。
Mol Cell. 2016 Mar 17;61(6):914-24. doi: 10.1016/j.molcel.2016.02.030.
2
Single-cell analysis tools for drug discovery and development.用于药物发现与开发的单细胞分析工具。
Nat Rev Drug Discov. 2016 Mar;15(3):204-16. doi: 10.1038/nrd.2015.16. Epub 2015 Dec 16.
3
Single-cell RNA-seq: advances and future challenges.单细胞RNA测序：进展与未来挑战
Nucleic Acids Res. 2014 Aug;42(14):8845-60. doi: 10.1093/nar/gku555. Epub 2014 Jul 22.
4
Single cell genomics: advances and future perspectives.单细胞基因组学：进展与未来展望。
PLoS Genet. 2014 Jan 30;10(1):e1004126. doi: 10.1371/journal.pgen.1004126. eCollection 2014 Jan.
5
The promise and challenge of high-throughput sequencing of the antibody repertoire.高通量测序抗体库的前景与挑战。
Nat Biotechnol. 2014 Feb;32(2):158-68. doi: 10.1038/nbt.2782. Epub 2014 Jan 19.
6
Transcriptome in vivo analysis (TIVA) of spatially defined single cells in live tissue.活组织中空间限定的单细胞转录组体内分析（TIVA）。
Nat Methods. 2014 Feb;11(2):190-6. doi: 10.1038/nmeth.2804. Epub 2014 Jan 12.
7
Single-cell sequencing-based technologies will revolutionize whole-organism science.基于单细胞测序的技术将彻底改变整个生物体科学。
Nat Rev Genet. 2013 Sep;14(9):618-30. doi: 10.1038/nrg3542. Epub 2013 Jul 30.
8
Single-cell transcriptomics reveals bimodality in expression and splicing in immune cells.单细胞转录组学揭示了免疫细胞中表达和剪接的双峰模式。
Nature. 2013 Jun 13;498(7453):236-40. doi: 10.1038/nature12172. Epub 2013 May 19.
9
An automated system for high-throughput single cell-based breeding.高通量基于单细胞的自动育种系统。
Sci Rep. 2013;3:1191. doi: 10.1038/srep01191. Epub 2013 Feb 1.
10
Quantitative PCR analysis of DNA, RNAs, and proteins in the same single cell.在单个细胞中同时进行 DNA、RNA 和蛋白质的定量 PCR 分析。
Clin Chem. 2012 Dec;58(12):1682-91. doi: 10.1373/clinchem.2012.191445. Epub 2012 Sep 26.
Zotero 插件