心脏死亡后猪供肝移植复温机灌注的氧合调控
Regulated Oxygenation of Rewarming Machine Perfusion for Porcine Donation After Cardiac Death Liver Transplantation.
作者信息
Morito N, Obara H, Matsuno N, Enosawa S, Watanabe K, Furukori M, Furukawa H
机构信息
Mechanical Engineering, Tokyo Metropolitan University, Tokyo, Japan.
Mechanical Engineering, Tokyo Metropolitan University, Tokyo, Japan.
出版信息
Transplant Proc. 2016 May;48(4):1244-6. doi: 10.1016/j.transproceed.2015.12.098.
INTRODUCTION
Machine perfusion (MP) is particularly expected to preserve and resuscitate an organ obtained from extended criteria donors or donation after cardiac death to expand the donated organ pool for organ transplantation. This method requires to be investigated an optimal preservation condition. The aim of this study is investigation of the optimal oxygenation conditions under rewarming MP (RMP).
METHODS
Porcine livers were perfused with an RMP system developed by our research group. All livers were procured under warm ischemia time of 60 minutes, and preserved in static cold storage for 2 hours, and perfused for 2 hours using the RMP. For group 1, the livers were supplied with oxygen constantly through perfusate. For group 2, the livers were supplied with oxygen increasingly with controlling flow rates and oxygen concentration. Effluent enzymes were obtained during perfusion preservation.
RESULTS
The average levels of alanine aminotransferase were lower in group 2 than in group 1 during RMP, and also decreasing the hepatic artery pressures after 60 minutes.
CONCLUSIONS
Regulated oxygenation of RMP has possibility to improve the graft preservation for liver transplantation.
引言
机器灌注（MP）尤其有望保存并复苏从边缘供体或心脏死亡后捐献的器官，以扩大器官移植的供体器官库。这种方法需要研究最佳的保存条件。本研究的目的是探究复温机器灌注（RMP）下的最佳氧合条件。
方法
用我们研究小组开发的RMP系统对猪肝进行灌注。所有肝脏均在60分钟的热缺血时间下获取，在静态冷藏中保存2小时，并使用RMP灌注2小时。对于第1组，通过灌注液持续向肝脏供氧。对于第2组，通过控制流速和氧浓度逐渐向肝脏供氧。在灌注保存期间获取流出酶。
结果
在RMP期间，第2组的丙氨酸转氨酶平均水平低于第1组，并且在60分钟后肝动脉压力也降低。
结论
RMP的调节氧合有可能改善肝移植的移植物保存。
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