Baysal Birol, İnce Ali Tüzün, Gültepe Bilge, Gücin Zuhal, Malya Fatma Ümit, Tozlu Mukaddes, Şentürk Hakan, Bağcı Pelin, Çelikel Çiğdem Ataizi, Aker Fügen, Özkara Selvinaz, Paşaoğlu Esra, Dursun Nevra, Özgüven Banu Yılmaz, Tunçel Deniz
Gastroenterology Department, Bezmialem Vakıf University, Istanbul, Turkey.
Gastroenterology Department, Bezmialem Vakıf University, Istanbul, Turkey.
Pancreatology. 2016 Sep-Oct;16(5):865-8. doi: 10.1016/j.pan.2016.06.005. Epub 2016 Jun 13.
About half of the world population is infected with Helicobacter pylori (H. pylori), a bacterium associated with gastric cancer and considered to be a risk factor for pancreatic ductal adenocarcinoma. Whether the bacterium is associated with intraductal papillary mucinous neoplasm, believed to be a precursor of pancreatic ductal adenocarcinoma, is unknown. The aim of this study was to investigate the presence of H. pylori DNA in tissue sections of intraductal papillary mucinous neoplasm.
The presence of H. pylori DNA was tested in a retrospective controlled study of formalin-fixed, paraffin-embedded pancreatic tissues from 24 patients who underwent surgery for intraductal papillary mucinous neoplasm. Histologically normal tissues surrounding neoplasms were used as control. H. pylori DNA was evaluated after deparaffinization, DNA extraction, and purification, and results were evaluated statistically.
Samples were collected from 13 males and 11 females with mean age 59 years (range 44-77), and consisted of 19 cases of main-duct and three cases of branched-duct intraductal papillary mucinous neoplasm. Two patients were diagnosed with pancreatic cancer and main-duct intraductal papillary mucinous neoplasm. H. pylori DNA was not detected either in intraductal papillary mucinous neoplasm tissue, or in surrounding normal tissue.
Although H. pylori has been implicated in pancreatic ductal adenocarcinoma, it may not play a key role in the development of intraductal papillary mucinous neoplasm.
全球约一半人口感染幽门螺杆菌（H. pylori），这种细菌与胃癌相关，被认为是胰腺导管腺癌的一个危险因素。该细菌是否与被认为是胰腺导管腺癌前体的导管内乳头状黏液性肿瘤相关尚不清楚。本研究的目的是调查导管内乳头状黏液性肿瘤组织切片中幽门螺杆菌DNA的存在情况。
在一项回顾性对照研究中，对24例接受导管内乳头状黏液性肿瘤手术的患者的福尔马林固定、石蜡包埋胰腺组织进行幽门螺杆菌DNA检测。肿瘤周围组织学正常的组织用作对照。脱蜡、DNA提取和纯化后评估幽门螺杆菌DNA，并对结果进行统计学评估。
样本来自13名男性和11名女性，平均年龄59岁（范围44 - 77岁），包括19例主胰管型和3例分支胰管型导管内乳头状黏液性肿瘤。两名患者被诊断为胰腺癌合并主胰管型导管内乳头状黏液性肿瘤。在导管内乳头状黏液性肿瘤组织或周围正常组织中均未检测到幽门螺杆菌DNA。
尽管幽门螺杆菌与胰腺导管腺癌有关，但它可能在导管内乳头状黏液性肿瘤的发生发展中不发挥关键作用。
