Zeng T, Chen L, Du X, Lai S J, Huang S P, Liu Y L, Lu L Z
Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural sciences, Hangzhou, 310021, China.
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
Anim Genet. 2016 Oct;47(5):606-9. doi: 10.1111/age.12457. Epub 2016 Jun 22.
Residual feed intake (RFI) is now considered a more reasonable metric to evaluate animal feed efficiency. In this study, the correlation between RFI and other feed efficiency traits was investigated and gene expression within the hypothalamus was determined in low RFI (LRFI) and high RFI (HRFI) ducks. Further, several hypothalamic neuropeptide genes were measured using quantitative real-time PCR. The mean feed intake value was 160 g/day, whereas the egg mass laid (EML) and body weight were approximately 62.4 g/day and 1.46 kg respectively. Estimates for heritability of RFI, feed conversion ratio (FCR) and feed intake were 0.26, 0.18 and 0.23 respectively. RFI is phenotypically positively correlated with feed intake and FCR (P < 0.01). The expression of neuropeptide Y (NPY) and neuropeptide Y receptor Y5 (NPY5R) mRNA was higher in HRFI ducks compared with LRFI ducks (P < 0.05), whereas that of proopiomelanocortin (POMC), melanocortin 4 receptor (MC4R) and cholecystokinin (CCK) was lower (P < 0.05). The mRNA expression of gonadotropin-releasing hormone 1 (luteinizing-releasing hormone) (GNRH1) and prolactin receptor (PRLR) was unchanged between LRFI and HRFI ducks. The results indicate that selection for LRFI could reduce feed intake without significant changes in EML, whereas selection on FCR will increase EML.
剩余采食量（RFI）现已被认为是评估动物饲料效率的更合理指标。在本研究中，研究了RFI与其他饲料效率性状之间的相关性，并测定了低RFI（LRFI）和高RFI（HRFI）鸭下丘脑内的基因表达。此外，使用定量实时PCR检测了几个下丘脑神经肽基因。平均采食量为160克/天，而产蛋量（EML）和体重分别约为62.4克/天和1.46千克。RFI、饲料转化率（FCR）和采食量的遗传力估计值分别为0. .26、0.18和0.23。RFI在表型上与采食量和FCR呈正相关（P < 0.01）。与LRFI鸭相比，HRFI鸭中神经肽Y（NPY）和神经肽Y受体Y5（NPY5R）mRNA的表达更高（P < 0.05），而阿黑皮素原（POMC）、黑皮质素4受体（MC4R）和胆囊收缩素（CCK）的表达则较低（P < 0.05）。LRFI和HRFI鸭之间促性腺激素释放激素1（促黄体生成素释放激素）（GNRH1）和催乳素受体（PRLR）的mRNA表达没有变化。结果表明，选择LRFI可以降低采食量，而EML无显著变化，而选择FCR则会增加EML。
