Möckl Leonhard, Fessele Claudia, Despras Guillaume, Bräuchle Christoph, Lindhorst Thisbe K
Department of Physical Chemistry, Ludwig Maximilian University Munich, Butenandtstrasse 11, 81377 Munich, Germany.
Otto Diels Institute of Organic Chemistry, Christiana Albertina University of Kiel, Otto-Hahn-Platz 3-4, 24098 Kiel, Germany.
Biochim Biophys Acta. 2016 Sep;1860(9):2031-6. doi: 10.1016/j.bbagen.2016.06.021. Epub 2016 Jun 21.
We investigated the properties of six Escherichia coli adhesion inhibitors under static and under flow conditions. On mannan-covered model substrates and under static conditions, all inhibitors were able to almost completely abolish lectin-mediated E. coli adhesion. On a monolayer of living human microvascular endothelial cells (HMEC-1), the inhibitors reduced adhesion under static conditions as well, but a large fraction of bacteria still managed to adhere even at highest inhibitor concentrations. In contrast, under flow conditions E. coli did not exhibit any adhesion to HMEC-1 not even at inhibitor concentrations where significant adhesion was detected under static conditions. This indicates that the presence of shear stress strongly affects inhibitor properties and must be taken into account when evaluating the potency of bacterial adhesion inhibitors.
