Derivation of the human embryonic stem cell line RCe012-A (RC-8).
作者信息
De Sousa P A, Tye B J, Bruce K, Dand P, Russell G, Collins D M, Greenshields A, Bradburn H, Downie J M, Bateman M, Courtney A
机构信息
Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh, EH16 4UX, UK; Centre for Clinical Brain Sciences, University of Edinburgh, UK; MRC Centre for Regenerative Medicine, University of Edinburgh, UK.
Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh, EH16 4UX, UK.
出版信息
Stem Cell Res. 2016 Mar;16(2):489-92. doi: 10.1016/j.scr.2016.02.033. Epub 2016 Feb 23.
The human embryonic stem cell line RCe012-A (RC-8) was derived from a frozen and thawed day 5 embryo cultivated to the blastocyst stage. The embryo was voluntarily donated as unsuitable and surplus to fertility requirements following ethics committee approved informed consent under licence from the UK Human Fertilisation and Embryology Authority. The cell line shows normal pluripotency marker expression and differentiation to the three germ layers in vitro. It has a normal 46XX female karyotype and microsatellite PCR identity, HLA and blood group typing data is available.
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