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通过长距离聚合酶链反应检测弥漫性大B细胞淋巴瘤患者的t(8;14) c-myc/IgH基因重排

Detection of t(8;14) c-myc/IgH gene rearrangement by long-distance polymerase chain reaction in patients with diffuse large B-cell lymphoma.

作者信息

Kiaei Arezoo, Onsori Habib, Alijani Aylar, Andalib Sasan, Ghorbian Saeid, Sakhinia Ebrahim

机构信息

Department of Biology, Tabriz Branch, Islamic Azad University Tabriz, Iran; Department of Biology, College of Science, Tabriz Branch, Islamic Azad University, Tabriz, Iran.

Department of Cell and Molecular Biology, Marand Branch, Islamic Azad University, Marand, Iran.

出版信息

Hematol Oncol Stem Cell Ther. 2016 Dec;9(4):141-146. doi: 10.1016/j.hemonc.2016.05.006. Epub 2016 Jun 23.

DOI:10.1016/j.hemonc.2016.05.006

PMID:27352258

Abstract

OBJECTIVE/BACKGROUND: Specific chromosomal translocations are found in human leukemias and lymphomas. These translocations are closely related to particular histological and immunological phenotypes. In Burkitt's lymphoma, translocation t(8;14)(q24;q32), which involves the c-myc gene (8q24) and the immunoglobulin heavy-chain (IgH) locus (14q32), accounts for 90-95% of all chromosomal translocations. This translocation can be found in 2-5% of diffuse large B-cell lymphoma (DLBCL). Long-distance polymerase chain reaction (LD-PCR) assays, which can identify oncogene/Ig gene rearrangement, can detect these fusion genes. The objective of this study was to detect t(8;14) c-myc/IgH gene rearrangement by LD-PCR in patients with DLBCL.

METHODS

In this study, 54 DLBCL cases were tested by LD-PCR with specific primers. LD-PCR was used for two breakpoints in both the IgH gene (joining region and γ switch region) and the myc gene (Exons 2 and 3).

RESULTS

As much as 1.85% of the samples were positive for the γ constant region and Exon 2 of the myc gene.

CONCLUSION

LD-PCR can be used for the detection of t(8;14) c-myc/IgH gene rearrangement in patients with DLBCL.

摘要

目的/背景：特定的染色体易位见于人类白血病和淋巴瘤。这些易位与特定的组织学和免疫学表型密切相关。在伯基特淋巴瘤中，涉及c-myc基因（8q24）和免疫球蛋白重链（IgH）基因座（14q32）的t(8;14)(q24;q32)易位占所有染色体易位的90 - 95%。这种易位在2 - 5%的弥漫性大B细胞淋巴瘤（DLBCL）中也可发现。能够识别癌基因/I g基因重排的长距离聚合酶链反应（LD-PCR）检测可检测到这些融合基因。本研究的目的是通过LD-PCR检测DLBCL患者中的t(8;14) c-myc/I gH基因重排。