Respiratory Unit, Department of Pediatrics, University Hospital of Alexandroupolis, Alexandroupolis, Thrace, Greece.
Respiratory Unit, Department of Pediatrics, Academic General Hospital of Alexandroupolis, 68100, Dragana Alexandroupolis, Thrace, Greece.
World J Pediatr. 2017 Feb;13(1):34-40. doi: 10.1007/s12519-016-0043-9. Epub 2016 Jun 15.
Childhood asthma phenotype is the consequence of interaction between environment and genetic factors. Nitric oxide (NO) formation is affected by polymorphisms in nitric oxide synthase (NOS) enzymes, which play a significant role as inflammatory factors in the airways. This study was undertaken to estimate the correlation of -786C>T and 894G>T polymorphisms of the eNOS gene with the sensitization of asthmatic children to common aeroallergens.
A total of 193 asthmatic children and 96 healthy controls, who were of Mediterranean origin, living in the same geographical area, were enrolled in the study. 894G>T and -786T/C polymorphisms of the eNOS gene were analyzed using a PCR-RFLP method.
The 894GG genotype was more frequent (68.6%) in children with asthma sensitized to Oleaeuropaea than in those with asthma non-sensitized (43.0%) (P=0.004). Likewise, -786TT genotype frequency was higher in children with asthma sensitized to Oleaeuropaea (51.0%) than in those with asthma nonsensitized (31.7%) to this allergen (P=0.035). For the aeroallergens Parietariajudaica and mixed grass, the frequency of -786C allele carriage was associated with protection from sensitization to Parietariajudaica and mixed grass in asthmatic children (P=0.021 and P=0.017, respectively). In the healthy control group, the genotype frequencies for these polymorphisms were similar to genotype frequencies of children with asthma non-sensitized to these three specific aeroallergens.
In children with asthma, 894G>T and -786T/C polymorphisms of the eNOS gene were correlated with sensitization to common seasonal aeroallergens.
儿童哮喘表型是环境和遗传因素相互作用的结果。一氧化氮（NO）的形成受一氧化氮合酶（NOS）酶的多态性影响，NOS 酶作为气道中的炎症因子发挥着重要作用。本研究旨在评估内皮型一氧化氮合酶（eNOS）基因-786C>T 和 894G>T 多态性与哮喘儿童对常见空气变应原致敏的相关性。
共纳入 193 例哮喘儿童和 96 例健康对照者，均为地中海地区人群，生活在同一地理区域。采用 PCR-RFLP 法分析 eNOS 基因 894G>T 和-786T/C 多态性。
与非 Oleaeuropaea 致敏哮喘儿童相比，Oleaeuropaea 致敏哮喘儿童中 894GG 基因型更为常见（68.6%比 43.0%，P=0.004）。同样，与非 Oleaeuropaea 致敏哮喘儿童相比，Oleaeuropaea 致敏哮喘儿童中-786TT 基因型频率更高（51.0%比 31.7%，P=0.035）。对于空气变应原豚草和混合草，-786C 等位基因携带频率与保护儿童免受对豚草和混合草的致敏相关（分别为 P=0.021 和 P=0.017）。在健康对照组中，这些多态性的基因型频率与对这三种特定空气变应原无致敏的哮喘儿童的基因型频率相似。
在哮喘儿童中，eNOS 基因 894G>T 和-786T/C 多态性与对常见季节性空气变应原的致敏相关。
