Seydoux G, Greenwald I
Department of Biology, Princeton University, New Jersey 08544.
Cell. 1989 Jun 30;57(7):1237-45. doi: 10.1016/0092-8674(89)90060-3.
The lin-12 gene of C. elegans encodes a predicted transmembrane protein that controls a decision by two cells, Z1.ppp and Z4.aaa, between the anchor cell (AC) and ventral uterine precursor cell (VU) fates. We performed laser ablation experiments to demonstrate that specification of the VU fate of Z1.ppp or Z4.aaa depends on an "AC-to-VU" signal from the presumptive AC. We generated genetic mosaics in which defined cells lacked lin-12 activity. By correlating the fates of Z1.ppp and Z4.aaa with the lin-12 genotype of nearly every cell in these mosaics, we conclude that lin-12 function is VU cell autonomous. We present a model in which lin-12 functions in the receiving mechanism for the "AC-to-VU" signal leading to the specification of the AC and VU fates of Z1.ppp and Z4.aaa.
秀丽隐杆线虫的lin-12基因编码一种预测的跨膜蛋白，该蛋白控制两个细胞Z1.ppp和Z4.aaa在锚定细胞（AC）命运和腹侧子宫前体细胞（VU）命运之间的抉择。我们进行了激光消融实验，以证明Z1.ppp或Z4.aaa的VU命运特化取决于来自假定AC的“AC到VU”信号。我们构建了特定细胞缺乏lin-12活性的基因嵌合体。通过将Z1.ppp和Z4.aaa的命运与这些嵌合体中几乎每个细胞的lin-12基因型相关联，我们得出结论，lin-12功能是VU细胞自主的。我们提出了一个模型，其中lin-12在“AC到VU”信号的接收机制中起作用，导致Z1.ppp和Z4.aaa的AC和VU命运特化。
