Hayashi Kouhei, Ikari Tatsuya, Sugiyama Goro, Sugiura Tsuyoshi, Ohyama Yukiko, Kumamaru Wataru, Shirasuna Kanemitsu, Mori Yoshihide
Section of Oral and Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical Science, Faculty of Dental Science, Kyushu University, Japan.
Maxillofacial Diagnostic and Surgical Sciences, Department of Oral and Maxillofacial Rehabilitation, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8520, Japan.
Biochem Biophys Res Commun. 2016 Sep 2;477(4):814-819. doi: 10.1016/j.bbrc.2016.06.140. Epub 2016 Jun 29.
The mouse submandibular gland (SMG) is important organ for embryonic development, and branching morphogenesis is regulated by many molecules containing transcription factors. Real-time reverse transcriptase polymerase chain reaction revealed that the expression of Brachyury increased in the SMG and peaked between E12.5-E13.5, concomitant with the early stage of branching morphogenesis. The expression of Brachyury in SMG rudiments between E12.5-E13.5 was confirmed by western blotting. In addition, fibronectin and Btbd7 (regulated by fibronectin), which are both essential for cleft formation, were expressed strongly during the same period. The Sox2 and Wnt3a, which regulate cell growth, were also expressed strongly during E12.5-E13.5. On the other hand, cleft formation and branching morphogenesis was suppressed by knockdown of Brachyury gene, suggesting that Brachyury plays a central role in regulating cell growth and cleft formation in early-stage embryonic mouse salivary gland development.
小鼠下颌下腺（SMG）是胚胎发育的重要器官，分支形态发生受许多分子（包括转录因子）调控。实时逆转录聚合酶链反应显示，短尾相关转录因子（Brachyury）在SMG中的表达增加，并在胚胎第12.5天至13.5天达到峰值，这与分支形态发生的早期阶段一致。通过蛋白质免疫印迹法证实了胚胎第12.5天至13.5天期间Brachyury在SMG原基中的表达。此外，对于裂隙形成至关重要的纤连蛋白和Btbd7（受纤连蛋白调控）在同一时期也强烈表达。调节细胞生长 的Sox2和Wnt3a在胚胎第12.5天至13.5天期间也强烈表达。另一方面，敲低Brachyury基因可抑制裂隙形成和分支形态发生，这表明Brachyury在调节早期胚胎小鼠唾液腺发育中的细胞生长和裂隙形成中起核心作用。
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