miR-26a对视网膜母细胞瘤细胞中Beclin 1表达的影响。
Effects of miR-26a on the expression of Beclin 1 in retinoblastoma cells.
作者信息
Li M, Chen X M, Wang D M, Gan L, Qiao Y
机构信息
Department of Ophthalmology, West China Hospital of Sichuan University, Chengdu, Sichuan, China.
Department of Ophthalmology, AVIC 363 Hospital of Chengdu, Chengdu, Sichuan, China.
出版信息
Genet Mol Res. 2016 Jul 14;15(2):gmr8193. doi: 10.4238/gmr.15028193.
The aim of this study was to evaluate the effects of miR-26a on Beclin 1 expression in retinoblastoma (RB) cell lines (Y79 and WERi-RB-1). RB cells were transfected with miR-26a mimic, antagomir-26a, or control mimic. The Beclin 1 mRNA and protein levels were detected by quantitative polymerase chain reaction and western blot, respectively. The activity of Beclin 1 3ꞌ-UTR reporter gene was detected with the luciferase assay. After transfection with miR-26a mimic, Beclin 1 mRNA and protein levels as well as the activity of the 3'-UTR reporter gene decreased. However, all were increased upon inhibition of miR-26a with antagomir-26a. Beclin 1 is the target of miR-26a in human RB cell lines Y79 and WERi-RB-1, and miR-26a inhibits the expression of Beclin 1 by reducing its mRNA and protein levels.
本研究旨在评估miR-26a对视网膜母细胞瘤（RB）细胞系（Y79和WERi-RB-1）中Beclin 1表达的影响。用miR-26a模拟物、抗miR-26a或对照模拟物转染RB细胞。分别通过定量聚合酶链反应和蛋白质印迹法检测Beclin 1的mRNA和蛋白质水平。用荧光素酶测定法检测Beclin 1 3'-UTR报告基因的活性。用miR-26a模拟物转染后，Beclin 1的mRNA和蛋白质水平以及3'-UTR报告基因的活性均降低。然而，用抗miR-26a抑制miR-26a后，所有指标均升高。在人RB细胞系Y79和WERi-RB-1中，Beclin 1是miR-26a的靶标，且miR-26a通过降低Beclin 1的mRNA和蛋白质水平来抑制其表达。
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