正常人淋巴细胞中的α-L-岩藻糖苷酶活性
Alpha-L-fucosidase activity in normal human lymphocytes.
作者信息
Roger L, Bernard M A, Percheron F, Foglietti M J
机构信息
Laboratoire de Chimie Biologique, U.E.R. de Biologie Humaine et Expérimentale, Université René Descartes, Paris, France.
出版信息
Clin Chim Acta. 1989 Apr 14;180(3):303-10. doi: 10.1016/0009-8981(89)90012-0.
After DEAE-Trisacryl chromatography two forms of alpha-L-fucosidase have been characterized in normal human lymphocytes. These enzymatic forms were different with respect to their optimum pH, kinetic properties and isoelectric behaviour. After neuraminidase treatment two forms are still observed with a neutral shift in pI values. These results suggest that at least two structurally different alpha-L-fucosidase units exist.
经DEAE-三羟甲基氨基甲烷-琼脂糖层析后，已在正常人淋巴细胞中鉴定出两种形式的α-L-岩藻糖苷酶。这些酶形式在最适pH值、动力学特性和等电行为方面有所不同。经神经氨酸酶处理后，仍观察到两种形式，其等电点值有中性偏移。这些结果表明至少存在两种结构不同的α-L-岩藻糖苷酶单位。
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