Little work has been done on the effect that continuous passive motion (CPM) may have on maintaining the mechanical and biochemical characteristics of tendons deprived of normal load-bearing stimuli. This study compared changes in mechanical properties and collagen concentrations of the tibialis anterior tendon between two groups of rabbits: control animals that received no treatment and experimental animals that received CPM to one ankle and immobilization to the other. Treatment duration was three weeks. Mechanical testing yielded values for elastic modulus, stiffness, stress, strain, and strain energy density at linear and maximum loads. Hydroxyproline concentrations were measured to determine tendon collagen content. Findings indicated that cyclic tensile loading of tendons by CPM lessened the harmful effects of immobilization. Linear load and stress for CPM and control tendons were similar, while the same measures for immobilized tendons were 16% and 25% less than control tendons. Ultimate strength of the control tendons, however, exceeded CPM tendons by 16% and immobilized tendons by 20%. Hydroxyproline levels were comparable for the CPM, control, and immobilized tissues. For a short duration (three weeks), CPM reduced the detrimental effects of immobilization in the tendon, primarily in the linear-load region.