甲状腺功能亢进大鼠棕色脂肪组织线粒体和微粒体的脂质组成

Lipid composition of brown adipose tissue mitochondria and microsomes in hyperthyroid rats.

Ruggiero F M, Gnoni G V, Quagliariello E

Department of Biochemistry and Molecular Biology, University of Bari, Italy.

Int J Biochem. 1989;21(3):327-32. doi: 10.1016/0020-711x(89)90191-2.

Abstract

The effects of triiodothyronine on the lipid composition of rat brown adipose tissue (BAT) mitochondria and microsomes was investigated by high performance liquid chromatography (HPLC). 2. An increase of about 20% was noted in mitochondrial cholesterol and phospholipids, while a decrease of about 20% for both total cholesterol and phospholipids was observed in microsomes from hyperthyroid rats. 3. The BAT phospholipid composition was altered significantly in mitochondria from T3-treated rats with an increase (41%) of cardiolipin and a decrease (18%) in phosphatidylcholine. 4. In microsomes, a decrease by 25% in phosphatidylinositol was accompanied by a similar additional percentage increase in phosphatidylethanolamine. 5. Important alterations in the fatty acid pattern were found in mitochondrial neutral lipids.

摘要

采用高效液相色谱法（HPLC）研究了三碘甲状腺原氨酸对大鼠棕色脂肪组织（BAT）线粒体和微粒体脂质成分的影响。2. 甲状腺功能亢进大鼠的微粒体中，总胆固醇和磷脂均下降约20%，而线粒体中的胆固醇和磷脂增加约20%。3. 用T3处理的大鼠线粒体中，BAT磷脂组成发生显著变化，心磷脂增加（41%），磷脂酰胆碱减少（18%）。4. 在微粒体中，磷脂酰肌醇减少25%，同时磷脂酰乙醇胺增加约相同比例。5. 在线粒体中性脂质中发现脂肪酸模式有重要变化。