生物有机分子辐射脱氨和脱磷酸作用中的比较自由基化学。I.无氧溶液。

Comparative free-radical chemistry in the radiolytic deamination and dephosphorylation of bio-organic molecules. I. Oxygen-free solutions.

作者信息

Garrison W M

机构信息

University of California, Berkeley, Lawrence Berkeley Laboratory, CA 94720.

出版信息

Free Radic Biol Med. 1989;6(3):285-8. doi: 10.1016/0891-5849(89)90055-5.

DOI:10.1016/0891-5849(89)90055-5

PMID:2744577

Abstract

A wide range of experimental data is evaluated in support of the hypothesis that the reductive deamination of amino acids and peptides by eaq- and the oxidative dephosphorylation of glycol phosphates by OH in oxygen free solution are related in terms of a common elimination reaction involving free-radical intermediates of the same genre, that is, XCH(R)C(OH)R----HX + CH(R)COR where X = NH2, RCONH, and H2PO4 respectively. The proposed reaction model unifies for the first time the basic free-radical chemistry of N-C and PO-C bond cleavage in the radiolysis of peptides and glycol phosphates including proteins and nucleic acids in oxygen-free solution.

摘要

评估了大量实验数据，以支持以下假设：在无氧溶液中，水合电子对氨基酸和肽的还原脱氨基作用以及羟基对磷酸酯的氧化脱磷酸作用，在涉及相同类型自由基中间体的共同消除反应方面是相关的，即XCH(R)C(OH)R→HX + CH(R)COR，其中X分别为NH2、RCONH和H2PO4。所提出的反应模型首次统一了无氧溶液中肽和磷酸酯（包括蛋白质和核酸）辐射分解中N-C和PO-C键断裂的基本自由基化学。