用于移植患者来源的原发性导管原位癌（DCIS）细胞和细胞系的小鼠乳腺导管内（MIND）方法
Mouse Mammary Intraductal (MIND) Method for Transplantation of Patient Derived Primary DCIS Cells and Cell Lines.
作者信息
Kittrell Frances, Valdez Kelli, Elsarraj Hanan, Hong Yan, Medina Daniel, Behbod Fariba
机构信息
Department of Cell Biology, Baylor College of Medicine, University of Kansas Medical School, Kansas City, USA.
Department of Pathology and Laboratory Medicine, University of Kansas Medical School, Kansas City, USA.
出版信息
Bio Protoc. 2016 Mar 5;6(5). doi: 10.21769/bioprotoc.1744.
The MIND method involves intraductal injection of patient derived ductal carcinoma in situ (DCIS) cells and DCIS cell lines (MCF10DCIS.COM and SUM225) inside the mouse mammary ducts [Video 1 and Figure 1 in Behbod (2009)]. This method mimics the normal environment of DCIS and facilitates study of the natural progression of human DCIS, ., their initial growth as carcinoma within the ducts, followed by invasion into the stroma through the myoepithelial cell layer and basement membrane (Behbod , 2009; Valdez , 2011). In order to demonstrate that transplantation procedure is successful, the transplanted mammary glands may be excised as early as two weeks following intraductal injection of cells followed by Hematoxylin and Eosin (H&E) staining and/or immunofluorescence staining using human specific cytokeratin 5 and/or 19 [please see Figures 2-4 in Behbod (2009)]. Additionally, the presence of trypan blue inside the mouse mammary ducts immediately following intraductal injection is the best indicator that the injection was successful (Video 1 starting at 4:33 sec).
MIND方法包括将患者来源的导管原位癌（DCIS）细胞和DCIS细胞系（MCF10DCIS.COM和SUM225）经导管注射到小鼠乳腺导管内[Behbod（2009年）的视频1和图1]。该方法模拟了DCIS的正常环境，便于研究人类DCIS的自然进展，即它们最初在导管内作为癌生长，随后通过肌上皮细胞层和基底膜侵入间质（Behbod，2009年；Valdez，2011年）。为了证明移植程序成功，在经导管注射细胞后最早两周可切除移植的乳腺，然后进行苏木精和伊红（H&E）染色和/或使用人特异性细胞角蛋白5和/或19进行免疫荧光染色[请参见Behbod（2009年）的图2 - 4]。此外，经导管注射后立即在小鼠乳腺导管内存在台盼蓝是注射成功的最佳指标（视频1从4:33秒开始）。
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