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ECM1通过诱导上皮-间质转化促进肝细胞癌的迁移和侵袭。

ECM1 promotes migration and invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition.

作者信息

Chen Hao, Jia Weidong, Li Jiansheng

机构信息

Department of Hepatic Surgery, Affiliated Provincial Hospital, Anhui Medical University, Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, 17 Lujiang Road, Hefei, 230001, Anhui Province, People's Republic of China.

出版信息

World J Surg Oncol. 2016 Jul 27;14(1):195. doi: 10.1186/s12957-016-0952-z.

DOI:10.1186/s12957-016-0952-z
PMID:27460906
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4962417/
Abstract

BACKGROUND

Extracellular matrix protein 1 (ECM1) is a glycoprotein involved in many biologic processes. To determine the expression of ECM1 in hepatocellular carcinoma (HCC), and to study the role of ECM1 in inducing epithelia-mesenchymal transition (EMT) to analyze the effect of ECM1 on the migration and invasion of HCC cells.

METHODS

The expression of ECM1 in HCC specimens was examined by immunohistochemistry staining, and the correlations were analyzed between the expression of ECM1 and the clinicopathological data. The ECM1 expression plasmid was transfected into Bel-7402 cells to induce exogenous overexpression of ECM1 protein. The changes of the expression of ECM1, EMT-related protein (E-cadherin, Vimentin), in Bel-7402 cells were detected by Western blot after transfection of ECM1; the wound healing and invasion assay in vitro were used to determine the role of ECM1 gene transfection on the ability of migration and invasive potential of Bel-7402 cells.

RESULTS

Immumohistochemistry staining method displayed the ECM1 expression was positively associated with vascular invasion, TNM stage, and poor prognosis. A significant positive correlation was found between the expressions of ECM1 and Vimentin. After ECM1 overexpression, Western blot exhibited that the expression of E-cadherin was down-regulated and Vimentin expression was up-regulated in Bel-7402 cells; the wound healing and invasion assay showed that the migration and invasion potentials of Bel-7402 cells were significantly enhanced.

CONCLUSIONS

ECM1, which displayed a high expression in HCC specimens, was closely associated with clinicopathologic data and may promote migration and invasion of HCC cells by inducing EMT.

摘要

背景

细胞外基质蛋白1（ECM1）是一种参与多种生物学过程的糖蛋白。旨在确定ECM1在肝细胞癌（HCC）中的表达，并研究ECM1在诱导上皮-间质转化（EMT）中的作用，以分析ECM1对HCC细胞迁移和侵袭的影响。

方法

采用免疫组织化学染色检测HCC标本中ECM1的表达，并分析ECM1表达与临床病理数据之间的相关性。将ECM1表达质粒转染至Bel-7402细胞，以诱导ECM1蛋白的外源性过表达。转染ECM1后，通过蛋白质免疫印迹法检测Bel-7402细胞中ECM1、EMT相关蛋白（E-钙黏蛋白、波形蛋白）表达的变化；采用体外伤口愈合和侵袭实验确定ECM1基因转染对Bel-7402细胞迁移能力和侵袭潜能的作用。

结果

免疫组织化学染色显示ECM1表达与血管侵犯、TNM分期及预后不良呈正相关。ECM1与波形蛋白的表达之间存在显著正相关。ECM1过表达后，蛋白质免疫印迹法显示Bel-7402细胞中E-钙黏蛋白表达下调，波形蛋白表达上调；伤口愈合和侵袭实验表明Bel-7402细胞的迁移和侵袭潜能显著增强。

结论

ECM1在HCC标本中高表达，与临床病理数据密切相关，可能通过诱导EMT促进HCC细胞的迁移和侵袭。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6b8/4962417/fa2d6edcd4df/12957_2016_952_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6b8/4962417/88db20f29a8d/12957_2016_952_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6b8/4962417/1410998f9fd6/12957_2016_952_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6b8/4962417/949846a9dec8/12957_2016_952_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6b8/4962417/ed339a9a2d6e/12957_2016_952_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6b8/4962417/e204d47cc038/12957_2016_952_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6b8/4962417/fa2d6edcd4df/12957_2016_952_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6b8/4962417/88db20f29a8d/12957_2016_952_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6b8/4962417/1410998f9fd6/12957_2016_952_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6b8/4962417/949846a9dec8/12957_2016_952_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6b8/4962417/ed339a9a2d6e/12957_2016_952_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6b8/4962417/e204d47cc038/12957_2016_952_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6b8/4962417/fa2d6edcd4df/12957_2016_952_Fig6_HTML.jpg

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