Xu Xiao-Huang, Zhang Le-Le, Wu Guo-Sheng, Chen Xin, Li Ting, Chen Xiuping, Wang Yi-Tao, Lu Jin-Jian
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.
Wuxi Medical School, Jiangnan University, Wuxi, Jiangsu, China.
Planta Med. 2017 Feb;83(3-04):254-260. doi: 10.1055/s-0042-113000. Epub 2016 Jul 27.
Solasodine, a steroidal alkaloid isolated from solanaceous species, exhibits anticancer activities on several cell lines. This study aimed to explore the antitumor potential of solasodine on ovarian cancer cells. The MTT assay, lactate dehydrogenase release assay, Hoechst 33342 staining, 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-imidacarbocyanine staining assay, and Annexin V/PI assay were conducted to investigate the antiproliferation and apoptosis-inducing effects of solasodine. Monodansylcadaverine staining was performed to label the acidic puncta on ovarian cancer HEY cells. A wound healing assay and Transwell assay were carried out to determine whether solasodine elicits an antimetastatic effect on HEY cells. A gelatin zymography assay was applied to detect the enzymatic activities of matrix metalloproteinases. Western blot was employed to examine relevant protein expression. Results revealed that solasodine inhibited cell viabilities in a time- and dose-dependent manner, triggered apoptotic body formation, reduced cell mitochondrial membrane potential, and interfered with autolysosome degradation in ovarian cancer cells. Solasodine also suppressed the migration and invasion of HEY cells by downregulating matrix metalloproteinase expression and activities. This study could be used as a basis for further studies on the molecular mechanisms of the antiproliferation, apoptosis-inducing, autophagy-modifying, and antimetastatic activities of solasodine.
茄解定是一种从茄科植物中分离出的甾体生物碱，对多种细胞系具有抗癌活性。本研究旨在探讨茄解定对卵巢癌细胞的抗肿瘤潜力。通过MTT法、乳酸脱氢酶释放法、Hoechst 33342染色法、5,5',6,6'-四氯-1,1',3,3'-四乙基-咪唑羰花青染色法和Annexin V/PI法来研究茄解定的抗增殖和诱导凋亡作用。采用单丹磺酰尸胺染色法标记卵巢癌HEY细胞上的酸性斑点。进行伤口愈合试验和Transwell试验以确定茄解定是否对HEY细胞产生抗转移作用。应用明胶酶谱法检测基质金属蛋白酶的酶活性。采用蛋白质免疫印迹法检测相关蛋白表达。结果显示，茄解定以时间和剂量依赖性方式抑制细胞活力，引发凋亡小体形成，降低细胞线粒体膜电位，并干扰卵巢癌细胞中的自溶酶体降解。茄解定还通过下调基质金属蛋白酶的表达和活性来抑制HEY细胞的迁移和侵袭。本研究可为进一步研究茄解定的抗增殖、诱导凋亡、自噬调节和抗转移活性的分子机制提供依据。
