Nardini Giordano, Origgi Francesco C, Leopardi Stefania, Zaghini Anna, Saunders Jimmy H, Vignoli Massimo
J Zoo Wildl Med. 2016 Jun;47(2):508-13. doi: 10.1638/2014-0217.1.
The aim of this study was to evaluate a large-core manual biopsy device (Spirotome(®), Medinvents, 3500 Hasselt, Belgium) for liver sampling and histologic diagnosis in green iguanas (Iguana iguana). The study included eight green iguanas, and two ultrasound-guided biopsies were collected for each lizard, for 16 biopsies in total. The procedure was carried out under general anesthesia induced by intravenous injection of propofol (10 mg/kg) maintained with a mixture of 2.0% isoflurane and 0.8-1.2 L/min oxygen after tracheal intubation. Fourteen (87.5%) of the 16 biopsies were considered diagnostic. Liver biopsy quality was assessed according to sample size and tissue preservation. In particular, mean length (16.2 ± 4.5 mm), width (2.2 ± 0.5 mm), area (34.8 ± 6.9 mm(2)), and number of portal areas (9.4 ± 3.9) of each biopsy were recorded for all green iguanas. The total available surface of the sections obtained from the biopsies and their grade of preservation enabled a satisfactory evaluation of the parenchymal architecture. One of the green iguanas in the study died the day after the procedure due to severe hemocoeloma. Risk assessment evaluation suggested that small green iguanas may not be suitable for this biopsy procedure.
本研究的目的是评估一种大型手动活检装置（Spirotome(®)，Medinvents，比利时哈瑟尔特3500）用于绿鬣蜥（Iguana iguana）肝脏取样和组织学诊断的效果。该研究纳入了8只绿鬣蜥，每只蜥蜴采集2次超声引导下活检样本，共16份活检样本。手术在静脉注射丙泊酚（10 mg/kg）诱导的全身麻醉下进行，气管插管后用2.0%异氟醚和0.8 - 1.2 L/min氧气的混合气体维持麻醉。16份活检样本中有14份（87.5%）被认为具有诊断价值。根据样本大小和组织保存情况评估肝脏活检质量。具体而言，记录了所有绿鬣蜥每份活检样本的平均长度（16.2±4.5 mm）、宽度（2.2±0.5 mm）、面积（34.8±6.9 mm²）和门管区数量（9.4±3.9）。从活检样本获得的切片的总可用表面及其保存等级能够对实质结构进行令人满意的评估。研究中的一只绿鬣蜥在手术后第二天因严重血胸死亡。风险评估表明，小型绿鬣蜥可能不适合这种活检程序。
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