用于功能研究的乳酸乳球菌膜蛋白生产

Membrane Protein Production in Lactococcus lactis for Functional Studies.

作者信息

Seigneurin-Berny Daphne, King Martin S, Sautron Emiline, Moyet Lucas, Catty Patrice, André François, Rolland Norbert, Kunji Edmund R S, Frelet-Barrand Annie

机构信息

Laboratoire de Physiologie Cellulaire et Végétale, CNRS (UMR-5168)/CEA/INRA (UMR1417)/Université Grenoble Alpes, BIG, CEA, Grenoble, France.

Medical Research Council, Mitochondrial Biology Unit, Hills Road, Cambridge, CB2 2XY, UK.

出版信息

Methods Mol Biol. 2016;1432:79-101. doi: 10.1007/978-1-4939-3637-3_6.

DOI:10.1007/978-1-4939-3637-3_6

PMID:27485331

Abstract

Due to their unique properties, expression and study of membrane proteins in heterologous systems remains difficult. Among the bacterial systems available, the Gram-positive lactic bacterium, Lactococcus lactis, traditionally used in food fermentations, is nowadays widely used for large-scale production and functional characterization of bacterial and eukaryotic membrane proteins. The aim of this chapter is to describe the different possibilities for the functional characterization of peripheral or intrinsic membrane proteins expressed in Lactococcus lactis.

摘要

由于其独特的性质，在异源系统中表达和研究膜蛋白仍然很困难。在现有的细菌系统中，传统上用于食品发酵的革兰氏阳性乳酸菌乳酸乳球菌，如今被广泛用于细菌和真核膜蛋白的大规模生产和功能表征。本章的目的是描述在乳酸乳球菌中表达的外周或内在膜蛋白功能表征的不同可能性。