Department of Biology, Faculty of Art and Science, University of Gaziantep, 27310 Gaziantep, Turkey.
Department of Food Engineering, Faculty of Engineering, University of Gaziantep, 27310 Gaziantep, Turkey.
Ecotoxicol Environ Saf. 2016 Nov;133:403-12. doi: 10.1016/j.ecoenv.2016.08.002. Epub 2016 Aug 7.
To the best of our knowledge, any study about biochemical response of filamentous algae in the complex freshwater ecosystems has not been found in the literature. This study was designed to explore biochemical response of filamentous algae in different water bodies from May 2013 to October 2014, using multivariate approach in the South East of Turkey. Environmental variables were measured in situ: water temperature, oxygen concentration, saturation, conductivity, salinity, pH, redox potential, and total dissolved solid. Chemical variables of aqueous samples and biochemical compounds of filamentous algae were also measured. It was found that geographic position and anthropogenic activities had strong effect on physico-chemical variables of water bodies. Variation in environmental conditions caused change in algal biomass composition due to the different response of filamentous species, also indicated by FTIR analysis. Biochemical responses not only changed from species to species, but also varied for the same species at different sampling time and sampling stations. Multivariate analyses showed that heavy metals, nutrients, and water hardness were found as the important variables governing the temporal and spatial succession and biochemical compounds. Nutrients, especially nitrate, could stimulate pigment and total protein production, whereas high metal content had adverse effects. Amount of malondialdehyde (MDA), H2O2, total thiol groups, total phenolic compounds, proline, total carbohydrate, and metal bioaccumulation by filamentous algae could be closely related with heavy metals in the ecosystems. Significant increase in MDA, H2O2, total thiol group, total phenolic compounds, and proline productions by filamentous algae and chlorosis phenomenon seemed to be an important strategy for alleviating environmental factors-induced oxidative stress as biomarkers.
据我们所知，目前还没有文献研究丝状藻类在复杂淡水生态系统中的生化反应。本研究旨在 2013 年 5 月至 2014 年 10 月期间，采用多元方法，在土耳其东南部，探讨不同水体中丝状藻类的生化反应。在现场测量环境变量：水温、氧浓度、饱和度、电导率、盐度、pH 值、氧化还原电位和总溶解固体。还测量了水样的化学变量和丝状藻类的生化化合物。研究发现，地理位置和人为活动对水体的理化变量有很强的影响。由于丝状物种的不同反应，环境条件的变化导致藻类生物量组成的变化，FTIR 分析也表明了这一点。生化反应不仅因物种而异，而且同一物种在不同采样时间和采样站也有所不同。多元分析表明，重金属、营养物质和水硬度是控制时空演替和生化化合物的重要变量。营养物质，特别是硝酸盐，可以刺激色素和总蛋白质的产生，而高金属含量则有不利影响。丝状藻类中的丙二醛（MDA）、H2O2、总巯基、总酚类化合物、脯氨酸、总碳水化合物和金属生物积累量可能与生态系统中的重金属密切相关。丝状藻类 MDA、H2O2、总巯基、总酚类化合物和脯氨酸产量的显著增加以及黄化现象似乎是一种重要的策略，可以缓解环境因素引起的氧化应激作为生物标志物。
