Amirifard Nasrin, Sadeghi Edris, Farshchian Negin, Haghparast Abbas, Choubsaz Mansour
Cancer Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran Email :
Asian Pac J Cancer Prev. 2016;17(7):3085-8.
Worldwide, colorectal cancer (CRC) is reported to be the fourth most common cancer in men and the third most common in women. KRAS is a protooncogene located on the short arm of chromosome 12. The aim of this study was to evaluate the KRAS oncogene and its relationship it with clinicopathologic features in 33 Kurdish patients.
Metastatic CRC between 2012 and 2016 that came to Imam Reza hospital, Kermanshah province, Iran, were analysed for KRAS mutations using allele specific PCR primers and pyrosequencing. Correlations between variables was analyzed in PASW SPSS and overall survival curves were plotted in Graph Pad prism 5.
The mean age for them at diagnosis was 51.5±12.6 years (range, 2276 years). Among the 33 patients that were sequenced, 12 samples in the KRAS gene had a nucleotide change, 11 in codon 12 and 1 in codon 13.There was no significant relationship between the mutation and clinical and pathological aspects of the disease.
Knowledge of the KRAS status can help in decisionmaking to treat metastatic colorectal cancer patients more efficiently and increase survival. However, many Kurdish people due to economic problems are not able to do this valuable genetic test. In addition, we need more careful research of KRAS oncogene at the molecular level in young populations with more patients.
据报道，在全球范围内，结直肠癌（CRC）是男性中第四大常见癌症，女性中第三大常见癌症。KRAS是一种位于12号染色体短臂上的原癌基因。本研究的目的是评估33名库尔德患者中KRAS癌基因及其与临床病理特征的关系。
对2012年至2016年间送至伊朗克尔曼沙阿省伊玛目礼萨医院的转移性结直肠癌患者，使用等位基因特异性PCR引物和焦磷酸测序分析KRAS突变情况。在PASW SPSS中分析变量之间的相关性，并在Graph Pad prism 5中绘制总生存曲线。
他们诊断时的平均年龄为51.5±12.6岁（范围，22至76岁）。在测序的33名患者中，KRAS基因的12个样本有核苷酸变化，其中11个在密码子12，1个在密码子13。该突变与疾病的临床和病理方面无显著关系。
了解KRAS状态有助于更有效地做出治疗转移性结直肠癌患者的决策并提高生存率。然而，许多库尔德人由于经济问题无法进行这项有价值的基因检测。此外，我们需要在更多患者的年轻人群中对KRAS癌基因进行更深入的分子水平研究。
