Pang S C
Department of Anatomy, Queen's University, Kingston, Ontario, Canada.
J Pathol. 1989 Jun;158(2):167-73. doi: 10.1002/path.1711580212.
The characteristics and proliferation of aortic smooth muscle cells (SMC) from spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) rats were studied in culture. Smooth muscle cells were isolated from the tunica media of the thoracic aorta by an explant method. Immunofluorescence microscopy showed that 93-95 per cent of cells were positively labelled with antibodies raised against smooth muscle actin, indicating that these were smooth muscle cells. The proliferative activity was compared between aortic smooth muscle cells from hypertensive and normotensive rats in culture by thymidine incorporation and cell number determinations. The results demonstrate that aortic smooth muscle cells from hypertensive rats grew faster than those from normotensive rats in culture. The increased proliferative activity of cultured aortic smooth muscle cells from hypertensive rats was detectable even when they were cultured in a chemically defined serum-free medium. These data have shown that an increased proliferative activity of aortic smooth muscle cells from hypertensive rats can occur in culture conditions without the influence of arterial pressure or other stimuli as in intact animals. The mechanisms underlying the accelerated proliferative activity of aortic smooth muscle cells from genetically hypertensive rats in vitro remain to be determined.
对自发性高血压大鼠（SHR）和正常血压的Wistar-Kyoto（WKY）大鼠的主动脉平滑肌细胞（SMC）的特性和增殖情况进行了体外研究。采用组织块培养法从胸主动脉中膜分离出平滑肌细胞。免疫荧光显微镜检查显示，93% - 95%的细胞被抗平滑肌肌动蛋白抗体阳性标记，表明这些是平滑肌细胞。通过掺入胸腺嘧啶核苷和测定细胞数量，比较了培养的高血压大鼠和正常血压大鼠的主动脉平滑肌细胞的增殖活性。结果表明，在体外培养条件下，高血压大鼠的主动脉平滑肌细胞比正常血压大鼠的生长更快。即使在化学限定的无血清培养基中培养，高血压大鼠培养的主动脉平滑肌细胞的增殖活性增加仍可检测到。这些数据表明，在体外培养条件下，高血压大鼠的主动脉平滑肌细胞增殖活性增加，且不受动脉血压或完整动物中其他刺激因素的影响。遗传性高血压大鼠主动脉平滑肌细胞增殖活性加速的潜在机制仍有待确定。
