由Bxb1整合酶催化的植物生物射弹定点整合方法。
Method for Biolistic Site-Specific Integration in Plants Catalyzed by Bxb1 Integrase.
作者信息
Li Ruyu, Han Zhiguo, Hou Lili, Kaur Gurminder, Yin Qian, Ow David W
机构信息
Plant Gene Engineering Center, South China Botanical Garden, Chinese Academy of Sciences, 723 Xingke Road, Guangzhou, 510650, China.
University of Chinese Academy of Sciences, Beijing, China.
出版信息
Methods Mol Biol. 2016;1469:15-30. doi: 10.1007/978-1-4939-4931-1_2.
Crop improvement is a never ending process. With a transgenesis approach, it is not inconceivable to envision a continuous addition of new transgenes to existing cultivars. Previously, we described a recombinase-directed gene stacking method in tobacco (Hou et al., Mol Plant 7:1756-1765, 2014). Being able to stack DNA to a previous location ensures that the number of genetic loci does not increase with each new round of transgene addition. Whereas the previous demonstration was conducted through polyethylene glycol to mediate uptake of DNA into tobacco protoplasts, we now describe protocols for using biolistic transformation to stack DNA in tobacco and rice.
作物改良是一个永无止境的过程。采用转基因方法，设想在现有品种中持续添加新的转基因并非不可想象。此前，我们描述了一种烟草中重组酶介导的基因叠加方法（Hou等人，《分子植物》7:1756 - 1765，2014年）。能够将DNA叠加到先前的位置可确保随着每一轮新的转基因添加，基因座数量不会增加。虽然之前的演示是通过聚乙二醇介导DNA进入烟草原生质体来进行的，但我们现在描述了使用生物弹道转化法在烟草和水稻中叠加DNA的方案。
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