Luo Weifeng, Nanjo Yohei, Komatsu Setsuko, Matsuura Hideyuki, Takahashi Kosaku
a Division of Fundamental Agriscience Research, Research Faculty of Agriculture , Hokkaido University , Sapporo , Japan.
b National Institute for Crop Science , National Agriculture and Food Research Organization , Tsukuba , Japan.
Biosci Biotechnol Biochem. 2016 Dec;80(12):2357-2364. doi: 10.1080/09168451.2016.1222268. Epub 2016 Aug 25.
12-Oxo-phytodienoic acid (OPDA) is induced by mechanical wounding and suppresses the growth of Physcomitrella patens; OPDA is considered as a signal compound in this moss species. In this study, a proteomic analysis of P. patens protonemata treated with OPDA was performed. The abundance levels of 41 proteins were significantly altered by OPDA, with decreased levels for 40 proteins. The proteins for which abundance decreased in response to OPDA at the protonema developmental stage were mainly involved in the metabolism of proteins and carbohydrates. The effects of inhibition on protein abundance are likely a major physiological function of OPDA in P. patens. OPDA also suppressed the expression of histones at the protein level and gene transcription level. Suppression of histone expression might be an OPDA-specific function in P. patens protonemata. In P. patens, a subset of the physiological responses caused by OPDA is shown to differ between protonema and gametophore developmental stages.
12-氧代植物二烯酸（OPDA）由机械损伤诱导产生，并抑制小立碗藓的生长；在这种苔藓植物中，OPDA被视为一种信号化合物。在本研究中，对用OPDA处理的小立碗藓原丝体进行了蛋白质组学分析。OPDA显著改变了41种蛋白质的丰度水平，其中40种蛋白质的水平降低。在原丝体发育阶段，响应OPDA而丰度降低的蛋白质主要参与蛋白质和碳水化合物的代谢。对蛋白质丰度的抑制作用可能是OPDA在小立碗藓中的主要生理功能。OPDA还在蛋白质水平和基因转录水平上抑制组蛋白的表达。组蛋白表达的抑制可能是OPDA在小立碗藓原丝体中的特异性功能。在小立碗藓中，由OPDA引起的一部分生理反应在原丝体和配子体发育阶段有所不同。
