酪氨酸激酶抑制剂苹果酸舒尼替尼单独及联合热疗对乳腺癌MCF-7细胞的体外细胞毒性作用。
In vitro cytotoxic effect of tyrosine kinase inhibitor sunitinib malate alone and in combination with hyperthermia on breast adenocarcinoma MCF-7 cells.
作者信息
Topcul Mehmet R, Cetin Idil
机构信息
Istanbul University, Faculty of Science, Department of Biology, Istanbul/Turkey.
出版信息
J BUON. 2016 May-Jun;21(3):556-63.
PURPOSE
In this study, the in vitro cytotoxic effect of sunitinib malate alone and combination with hyperthermia was evaluated on MCF-7 cells (human breast adenocarcinoma cell line).
METHODS
For this purpose cell proliferation assay, mitotic index and labelling index analysis among cell kinetic parameters were assessed. Sunitinib malate doses of 1, 5 and 10 μM were applied alone and in combination with hyperthermia to cells for 24-72 hrs.
RESULTS
A significant decrease (p<0.05) was noticed in cell proliferation, mitotic index and labelling index for all experimental groups and for all applications.
CONCLUSION
Labeling index and mitotic index values show that sunitinib malate combined with hyperthermia was significantly more effective in MCF-7 cells than when given alone. This combination acts through synergistic and additive effects.
目的
在本研究中，评估了苹果酸舒尼替尼单独使用以及与热疗联合使用对MCF - 7细胞（人乳腺腺癌细胞系）的体外细胞毒性作用。
方法
为此，评估了细胞增殖测定、细胞动力学参数中的有丝分裂指数和标记指数分析。将1、5和10 μM的苹果酸舒尼替尼剂量单独以及与热疗联合应用于细胞24 - 72小时。
结果
所有实验组和所有应用中，细胞增殖、有丝分裂指数和标记指数均显著降低（p<0.05）。
结论
标记指数和有丝分裂指数值表明，苹果酸舒尼替尼与热疗联合使用在MCF - 7细胞中比单独使用时显著更有效。这种联合作用通过协同和相加效应发挥作用。
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