Hung Jui-Hung, Weng Zhiping
Cold Spring Harb Protoc. 2017 Mar 1;2017(3):pdb.top093179. doi: 10.1101/pdb.top093179.
Microarray and next-generation sequencing technologies have greatly expedited the discovery of genomic DNA that can be enriched using various biochemical methods. Chromatin immunoprecipitation (ChIP) is a general method for enriching chromatin fragments that are specifically recognized by an antibody. The resulting DNA fragments can be assayed by microarray (ChIP-chip) or sequencing (ChIP-seq). This introduction focuses on ChIP-seq data analysis. The first step of analyzing ChIP-seq data is identifying regions in the genome that are enriched in a ChIP sample; these regions are called peaks.
微阵列和新一代测序技术极大地加速了可通过各种生化方法富集的基因组DNA的发现。染色质免疫沉淀（ChIP）是富集被抗体特异性识别的染色质片段的常用方法。所得的DNA片段可通过微阵列（ChIP芯片）或测序（ChIP测序）进行检测。本介绍重点关注ChIP测序数据分析。分析ChIP测序数据的第一步是识别基因组中在ChIP样本中富集的区域；这些区域称为峰。
