采用Chromosorb P柱进行样品制备，同时用液相色谱法测定某些支气管扩张剂、抗惊厥药、氯霉素和催眠药。

Simultaneous liquid-chromatographic determination of some bronchodilators, anticonvulsants, chloramphenicol, and hypnotic agents, with Chromosorb P columns used for sample preparation.

作者信息

Svinarov D A, Dotchev D C

机构信息

Clinical Laboratory, Medical Academy, Sofia, Bulgaria.

出版信息

Clin Chem. 1989 Aug;35(8):1615-8.

PMID:2758629

Abstract

We describe a simple liquid-chromatographic system for simultaneously measuring bronchodilators, anticonvulsants, hypnotics, and chloramphenicol. Use in therapeutic drug monitoring includes determination of theophylline, caffeine, chloramphenicol, ethosuximide, primidone, phenobarbital, phenacemide, phenytoin, mephenytoin, nirvanol, and carbamazepine and its bioactive metabolites within 13 min. In the "toxicology mode" theophylline, caffeine, barbital, butabarbital, pentobarbital, amobarbital, secobarbital, primidone, phenobarbital, methylprylon, glutethimide, methaqualone, phenytoin, mephenytoin, nirvanol, and carbamazepine and its bioactive metabolites are resolved within 17 min. A reversed-phase C8 column (5-microns particles) is used, with acetonitrile/water (20/80 by vol) as mobile phase. The drugs are extracted from 50 microL of serum with use of a Chromosorb P microcolumn and chloroform/isopropanol (6/1 by vol). The drugs are quantified by absorbance at 208 nm, with tolylphenobarbital as internal standard. Lower limits of detection varied from 0.05 to 0.1 mg/L, analytical recovery from 94% to 106%; CVs were less than 5.6% within run, less than 6.9% between runs.

摘要

我们描述了一种简单的液相色谱系统，用于同时测定支气管扩张剂、抗惊厥药、催眠药和氯霉素。其在治疗药物监测中的应用包括在13分钟内测定茶碱、咖啡因、氯霉素、乙琥胺、扑米酮、苯巴比妥、苯乙酰脲、苯妥英、甲妥英、尼凡诺和卡马西平及其生物活性代谢物。在“毒理学模式”下，茶碱、咖啡因、巴比妥、丁巴比妥、戊巴比妥、异戊巴比妥、司可巴比妥、扑米酮、苯巴比妥、甲普龙、格鲁米特、甲喹酮、苯妥英、甲妥英、尼凡诺和卡马西平及其生物活性代谢物可在17分钟内分离。使用反相C8柱（5微米颗粒），以乙腈/水（体积比20/80）作为流动相。使用Chromosorb P微柱和氯仿/异丙醇（体积比6/1）从50微升血清中提取药物。以甲苯基苯巴比妥作为内标，通过在208nm处的吸光度对药物进行定量。检测下限为0.05至0.1mg/L，分析回收率为94%至106%；批内变异系数小于5.6%，批间变异系数小于6.9%。