Baker Suzanne L, Lockhart Samuel N, Price Julie C, He Mark, Huesman Ronald H, Schonhaut Daniel, Faria Jamie, Rabinovici Gil, Jagust William J
Molecular Biophysics and Integrated Bioimaging, Lawrence Berkeley National Lab, Berkeley, California
Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, California.
J Nucl Med. 2017 Feb;58(2):332-338. doi: 10.2967/jnumed.116.175273. Epub 2016 Sep 1.
The goal of this paper was to evaluate the in vivo kinetics of the novel tau-specific PET radioligand F-AV-1451 in cognitively healthy control (HC) and Alzheimer disease (AD) subjects, using reference region analyses.
F-AV-1451 PET imaging was performed on 43 subjects (5 young HCs, 23 older HCs, and 15 AD subjects). Data were collected from 0 to 150 min after injection, with a break from 100 to 120 min. T1-weighted MR images were segmented using FreeSurfer to create 14 bilateral regions of interest (ROIs). In all analyses, cerebellar gray matter was used as the reference region. Nondisplaceable binding potentials (BPs) were calculated using the simplified reference tissue model (SRTM) and SRTM2; the Logan graphical analysis distribution volume ratio (DVR) was calculated for 30-150 min (DVR30-150). These measurements were compared with each other and used as reference standards for defining an appropriate 20-min window for the SUV ratio (SUVR). Pearson correlations were used to compare the reference standards to 20-min SUVRs (start times varied from 30 to 130 min), for all values, for ROIs with low F-AV-1451 binding (lROIs, mean of BP + 1 and DVR30-150 < 1.5), and for ROIs with high F-AV-1451 binding (hROIs, mean of BP + 1 and DVR30-150 > 1.5).
SRTM2 BP + 1 and DVR30-150 were in good agreement. Both were in agreement with SRTM BP + 1 for lROIs but were greater than SRTM BP + 1 for hROIs, resulting in a nonlinear relationship. hROI SUVRs increased from 80-100 to 120-140 min by 0.24 ± 0.15. The SUVR time interval resulting in the highest correlation and slope closest to 1 relative to the reference standards for all values was 120-140 min for hROIs, 60-80 min for lROIs, and 80-100 min for lROIs and hROIs. There was minimal difference between methods when statistical significance between ADs and HCs was calculated.
Despite later time periods providing better agreement between reference standards and SUVRs for hROIs, a good compromise for studying lROIs and hROIs is SUVR80-100. The lack of SUVR plateau for hROIs highlights the importance of precise acquisition time for longitudinal assessment.
本文的目的是使用参考区域分析方法，评估新型tau特异性正电子发射断层显像（PET）放射性配体F-AV-1451在认知健康对照（HC）和阿尔茨海默病（AD）受试者体内的动力学情况。
对43名受试者（5名年轻HC、23名年长HC和15名AD受试者）进行了F-AV-1451 PET成像。在注射后0至150分钟收集数据，中间在100至120分钟有中断。使用FreeSurfer对T1加权磁共振图像进行分割，以创建14个双侧感兴趣区域（ROI）。在所有分析中，小脑灰质用作参考区域。使用简化参考组织模型（SRTM）和SRTM2计算不可置换结合电位（BP）；计算30至150分钟的洛根图形分析分布容积比（DVR）（DVR30-150）。将这些测量值相互比较，并用作定义SUV比（SUVR）合适20分钟窗口的参考标准。使用Pearson相关性将参考标准与20分钟SUVR（开始时间从30到130分钟不等）进行比较，针对所有值、F-AV-1451结合低的ROI（低结合ROI，BP + 1和DVR30-150的平均值 < 1.5）以及F-AV-1451结合高的ROI（高结合ROI，BP + 1和DVR30-150的平均值 > 1.5）。
SRTM2 BP + 1和DVR30-150高度一致。对于低结合ROI，两者均与SRTM BP + 1一致，但对于高结合ROI，它们大于SRTM BP + 1，导致非线性关系。高结合ROI的SUVR在80 - 100至120 - 140分钟增加了0.24 ± 0.15。相对于所有值的参考标准，导致最高相关性且斜率最接近1的SUVR时间间隔，对于高结合ROI为120 - 140分钟，对于低结合ROI为60 - 80分钟，对于低结合ROI和高结合ROI均为80 - 100分钟。在计算AD和HC之间的统计学显著性时，各方法之间差异极小。
尽管较晚的时间段在参考标准和高结合ROI的SUVR之间提供了更好的一致性，但对于研究低结合ROI和高结合ROI而言，SUVR80-100是一个不错的折衷选择。高结合ROI缺乏SUVR平台期突出了精确采集时间对于纵向评估的重要性。
