在未孕母羊子宫颈内施用促卵泡激素（FSH）或促黄体生成素（LH）对子宫颈中透明质酸、环氧化酶2（COX2）及COX2信使核糖核酸（mRNA）水平的影响。
The effect of the intracervical administration of FSH or LH on the levels of hyaluronan, COX2, and COX2 mRNA in the cervix of the nonpregnant ewe.
作者信息
Leethongdee Sukanya, Khalid Muhammad, Scaramuzzi Rex J
机构信息
The Reproduction in Domestic Animal research Unit, Faculty of Veterinary Science, Mahasarakham University, Muang, Mahasarakham, Thailand; Department of Production and Population Health, The Royal Veterinary College, University of London, Hertfordshire, UK; Department of Comparative Biomedical Sciences, The Royal Veterinary College, University of London, Hertfordshire, UK.
Department of Production and Population Health, The Royal Veterinary College, University of London, Hertfordshire, UK.
出版信息
Theriogenology. 2016 Dec;86(9):2244-2253. doi: 10.1016/j.theriogenology.2016.07.014. Epub 2016 Jul 25.
During the periovulatory period, the cervix relaxes in response to changes in circulating concentrations of reproductive hormones. The present study investigated the role of gonadotrophins in cervical function by examining the expression of cyclooxygenase-2 (COX2) and COX2 mRNA and the concentration of hyaluronan (HA) in the cervix, after intracervical treatment with either FSH or LH. Eighteen ewes were assigned to four groups. They were then treated with commercial intravaginal progestagen sponges and eCG to synchronize their estrous cycles. Intracervical treatments were given 24 hours after removal of the sponges as follows: group 1: FSH, 2 mg; group 2: LH, 2 mg; group 3: vehicle; and group 4: control. Cervices were collected 54 hours after sponge removal and then divided into three regions. The expression of COX2 and COX2 mRNA was determined by immunohistochemistry and in situ hybridization and those of HA by ELISA. The levels of expression of COX2, COX2 mRNA, and HA were compared in six tissue layers (luminal epithelium, subepithelial stroma, circular, longitudinal and transverse muscle, and serosa) and in three cervical regions (vaginal, mid, and uterine). The results showed that both FSH and LH significantly increased the levels the COX2 mRNA and COX2 in the cervix, but the effects of the gonadotrophins were selective. The effects of both FSH and LH were most evident at the vaginal end of the cervix and least at the uterine end of the cervix. Furthermore, their effects were confined to the stroma and smooth muscle layers of the cervix in the case of FSH and to smooth muscle only in the case of LH. Neither FSH nor LH affected the concentration of HA in the cervix although FSH but not LH reduced the concentration of HA in cervical mucus. These findings suggest that the gonadotrophins regulate the expression of COX2 in the cervix and that they may have a role facilitating relaxation of the cervix during estrus in the ewe.
在排卵期前后，子宫颈会因循环生殖激素浓度的变化而松弛。本研究通过检测用促卵泡素（FSH）或促黄体生成素（LH）进行宫颈内治疗后，子宫颈中环氧合酶-2（COX2）和COX2信使核糖核酸（mRNA）的表达以及透明质酸（HA）的浓度，来研究促性腺激素在宫颈功能中的作用。18只母羊被分为四组。然后用商业阴道内孕激素海绵和孕马血清促性腺激素（eCG）对它们进行处理，以使它们的发情周期同步。在取出海绵24小时后进行宫颈内治疗，如下：第1组：FSH，2毫克；第2组：LH，2毫克；第3组：赋形剂；第4组：对照。在取出海绵54小时后收集子宫颈，然后将其分为三个区域。通过免疫组织化学和原位杂交测定COX2和COX2 mRNA的表达，通过酶联免疫吸附测定（ELISA）测定HA的表达。比较了六个组织层（管腔上皮、上皮下基质、环形、纵向和横向肌肉以及浆膜）和三个宫颈区域（阴道、中部和子宫部）中COX2、COX2 mRNA和HA的表达水平。结果表明，FSH和LH均显著提高了子宫颈中COX2 mRNA和COX2的水平，但促性腺激素的作用具有选择性。FSH和LH的作用在子宫颈的阴道端最明显，在子宫颈的子宫端最不明显。此外，FSH的作用局限于子宫颈的基质和平滑肌层，而LH的作用仅局限于平滑肌。FSH和LH均未影响子宫颈中HA的浓度，尽管FSH而非LH降低了宫颈黏液中HA的浓度。这些发现表明，促性腺激素调节子宫颈中COX2的表达，并且它们可能在母羊发情期间促进子宫颈松弛中发挥作用。
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