胍丁胺抑制微小RNA let-7a表达调控神经干细胞分化。
Suppression of MicroRNA let-7a Expression by Agmatine Regulates Neural Stem Cell Differentiation.
作者信息
Song Juhyun, Oh Yumi, Kim Jong Youl, Cho Kyoung Joo, Lee Jong Eun
机构信息
Department of Anatomy, Yonsei University College of Medicine, Seoul, Korea.
Brain Korea 21 Plus Project for Medical Sciences and Brain Research Institute, Yonsei University College of Medicine, Seoul, Korea.
出版信息
Yonsei Med J. 2016 Nov;57(6):1461-7. doi: 10.3349/ymj.2016.57.6.1461.
PURPOSE
Neural stem cells (NSCs) effectively reverse some severe central nervous system (CNS) disorders, due to their ability to differentiate into neurons. Agmatine, a biogenic amine, has cellular protective effects and contributes to cellular proliferation and differentiation in the CNS. Recent studies have elucidated the function of microRNA let-7a (let-7a) as a regulator of cell differentiation with roles in regulating genes associated with CNS neurogenesis.
MATERIALS AND METHODS
This study aimed to investigate whether agmatine modulates the expression of crucial regulators of NSC differentiation including DCX, TLX, c-Myc, and ERK by controlling let-7a expression.
RESULTS
Our data suggest that high levels of let-7a promoted the expression of TLX and c-Myc, as well as repressed DCX and ERK expression. In addition, agmatine attenuated expression of TLX and increased expression of ERK by negatively regulating let-7a.
CONCLUSION
Our study therefore enhances the present understanding of the therapeutic potential of NSCs in CNS disorders.
目的
神经干细胞（NSCs）因其分化为神经元的能力，可有效逆转一些严重的中枢神经系统（CNS）疾病。胍丁胺作为一种生物胺，具有细胞保护作用，并有助于中枢神经系统中的细胞增殖和分化。最近的研究阐明了微小RNA let-7a（let-7a）作为细胞分化调节因子的功能，其在调节与中枢神经系统神经发生相关的基因中发挥作用。
材料与方法
本研究旨在探讨胍丁胺是否通过控制let-7a的表达来调节神经干细胞分化关键调节因子（包括双皮质素（DCX）、同源框蛋白TLX（TLX）、原癌基因c-Myc（c-Myc）和细胞外信号调节激酶（ERK））的表达。
结果
我们的数据表明，高水平的let-7a促进了TLX和c-Myc的表达，同时抑制了DCX和ERK的表达。此外，胍丁胺通过负向调节let-7a来减弱TLX的表达并增加ERK的表达。
结论
因此，我们的研究增进了目前对神经干细胞在中枢神经系统疾病中治疗潜力的理解。
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