基于病毒样颗粒的家蚕蛹抗兔出血症病毒疫苗的研制
Development of a VLP-based vaccine in silkworm pupae against rabbit hemorrhagic disease virus.
作者信息
Zheng Xuexing, Wang Shukun, Zhang Weijiao, Liu Xingjian, Yi Yongzhu, Yang Songtao, Xia Xianzhu, Li Yinv, Zhang Zhifang
机构信息
School of Public Health, Shandong University, Jinan 250012, China; China Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 10081, China.
China Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 10081, China.
出版信息
Int Immunopharmacol. 2016 Nov;40:164-169. doi: 10.1016/j.intimp.2016.08.016. Epub 2016 Sep 3.
Rabbit hemorrhagic disease virus (RHDV) is the etiological agent behind rabbit hemorrhagic disease (RHD), which is lethal and contagious in rabbits. The virus does not replicate in cell culture and the only commercial inactivated vaccine available is derived from infected rabbit livers. RHDV capsid protein, VP60, is the main antigen comprising the virion. We used a baculovirus-silkworm pupae system to express VP60, which self-assembled into virus-like particles (VLPs) with a similar size and morphology to RHDV. Hemagglutination assays (HAs) showed that VP60 expression levels of VP60 reached as high as 10HA units (HAU) per pupa. A single intramuscular injection with 10HAU of VLPs completely protected rabbits for at least 180days against RHDV challenge, and for at least 360days when the VLPs were emulsified with Freund's complete adjuvant. These data suggest that silkworm pupae can be used to develop VLP-based vaccines which confer durable protection against RHD.
兔出血症病毒（RHDV）是兔出血症（RHD）的病原体，该病对兔具有致死性和传染性。该病毒不能在细胞培养物中复制，唯一可用的商业灭活疫苗来源于感染兔的肝脏。RHDV衣壳蛋白VP60是构成病毒粒子的主要抗原。我们利用杆状病毒-蚕蛹系统表达VP60，其自组装成大小和形态与RHDV相似的病毒样颗粒（VLPs）。血凝试验（HAs）表明，每个蛹的VP60表达水平高达10个血凝单位（HAU）。单次肌肉注射10HAU的VLPs可使兔子在至少180天内完全抵御RHDV攻击，当VLPs与弗氏完全佐剂乳化时，可使兔子在至少360天内得到保护。这些数据表明，蚕蛹可用于开发基于VLP的疫苗，该疫苗可对RHD提供持久保护。
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