Fan Jie, Ping Jie, Zhang Wan-Xia, Rao Yi-Song, Liu Han-Xiao, Zhang Jing, Yan You-E
a Department of Pharmacology , Basic Medical School of Wuhan University , Wuhan , China.
b The Center for Animal Experiment/Animal Biosafety Level 3 Laboratory , Wuhan University , Wuhan , China.
Ultrastruct Pathol. 2016 Sep-Oct;40(5):288-95. doi: 10.1080/01913123.2016.1223243. Epub 2016 Sep 6.
The aim of this study was to investigate the effects of prenatal and lactation nicotine exposure on the morphology and function of brown adipose tissue (BAT) in male rat offspring. We conducted a morphological assay and gene expression study of interscapular BAT (iBAT) in male rat offspring. The male offspring from nicotine-exposed dams exhibited higher body weight and iBAT weight. Hematoxylin and eosin staining and transmission electron microscopy showed that iBAT from nicotine-exposed male offspring presented a "whitening" phenotype characterized by lipid droplet accumulation and impaired mitochondria with a randomly oriented and fractured cristae. The expression of the iBAT structure and function-related genes all decreased in nicotine-exposed male offspring. These data indicate that prenatal and lactation nicotine exposure affects morphology and function of iBAT in male rat offspring.
本研究的目的是探讨产前和哺乳期尼古丁暴露对雄性大鼠后代棕色脂肪组织（BAT）形态和功能的影响。我们对雄性大鼠后代的肩胛间BAT（iBAT）进行了形态学分析和基因表达研究。来自尼古丁暴露母鼠的雄性后代体重和iBAT重量更高。苏木精和伊红染色以及透射电子显微镜显示，尼古丁暴露雄性后代的iBAT呈现出“变白”表型，其特征为脂滴积累和线粒体受损，嵴随机排列且断裂。尼古丁暴露雄性后代中iBAT结构和功能相关基因的表达均降低。这些数据表明，产前和哺乳期尼古丁暴露会影响雄性大鼠后代iBAT的形态和功能。
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