Xu Wangdong, Liu Yi, Ye Dongqing
a Department of Rheumatology and Immunology , West China Hospital, Sichuan University , Chengdu , Sichuan , PR China.
b Department of Epidemiology and Biostatistics , School of Public Health, Anhui Medical University , Hefei , Anhui , PR China.
Immunol Invest. 2016 Oct;45(7):575-83. doi: 10.1080/08820139.2016.1193868. Epub 2016 Sep 7.
Interleukin-33 (IL-33) is a member of the IL-1 family, and previous studies found the single-nucleotide polymorphisms (SNPs) in the IL-33 gene was related to susceptibility to autoimmune diseases, including rheumatoid arthritis, ankylosing spondylitis and Behcet's disease. To date, no study has discussed the potential association between IL-33 gene polymorphisms and systemic lupus erythematosus (SLE).
We conducted a case-control study including 371 SLE patients and 408 healthy controls to investigate the correlation between the SNPs of IL-33 gene (rs1929992, rs7044343) and SLE in a Chinese Han population.
There was significantly lower expression of allele G for rs1929992 in SLE patients than that in controls (G versus A, P = 0.012, OR = 1.310, 95% CI: 1.060-1.624 after adjustment with sex). Similarly, genotype GG was associated with the susceptibility to SLE as compared with the AA genotype (P = 0.017, OR = 1.714, 95% CI: 1.101-2.669 after adjustment with sex). We also found statistical significance in the dominant model (GG+GA versus AA, P = 0.017, OR = 1.481, 95% CI: 1.074-2.044 after adjustment with sex). However, we found no strong evidence for the association of IL-33 rs7044343 polymorphism with SLE. Moreover, association studies were performed on the relationship between the IL-33 gene polymorphisms and lupus nephritis as well as nine clinical features of SLE, but there was no significant association regarding the distribution of allele and genotype frequencies between SLE patients positive and negative for the presence of sub-phenotypes.
Our findings indicate that IL-33 rs1929992 polymorphism may be a potential biomarker for susceptibility to SLE.
白细胞介素-33（IL-33）是白细胞介素-1家族的成员，既往研究发现IL-33基因中的单核苷酸多态性（SNP）与自身免疫性疾病的易感性相关，包括类风湿关节炎、强直性脊柱炎和白塞病。迄今为止，尚无研究探讨IL-33基因多态性与系统性红斑狼疮（SLE）之间的潜在关联。
我们进行了一项病例对照研究，纳入371例SLE患者和408例健康对照，以调查中国汉族人群中IL-33基因（rs1929992、rs7044343）的SNP与SLE之间的相关性。
SLE患者中rs1929992的等位基因G表达明显低于对照组（G对A，P = 0.012，校正性别后OR = 1.310，95%CI：1.060 - 1.624）。同样，与AA基因型相比，基因型GG与SLE易感性相关（P = 0.017，校正性别后OR = 1.714，95%CI：1.101 - 2.669）。我们在显性模型中也发现了统计学意义（GG + GA对AA，P = 0.017，校正性别后OR = 1.481，95%CI：1.074 - 2.044）。然而，我们没有发现IL-33 rs7044343多态性与SLE相关的有力证据。此外，我们对IL-33基因多态性与狼疮性肾炎以及SLE的九个临床特征之间的关系进行了关联研究，但在亚表型存在与否的SLE患者阳性和阴性之间，等位基因和基因型频率分布没有显著关联。
我们的研究结果表明，IL-33 rs1929992多态性可能是SLE易感性的潜在生物标志物。
