一种用于快速常规鉴定PBP2a阳性金黄色葡萄球菌和凝固酶阴性葡萄球菌的商业免疫层析检测方法的评估。
Evaluation of a commercial immunochromatographic assay for rapid routine identification of PBP2a-positive Staphylococcus aureus and coagulase-negative staphylococci.
作者信息
Dupieux Céline, Trouillet-Assant Sophie, Tasse Jason, Freydière Anne-Marie, Raulin Olivia, Roure-Sobas Chantal, Salord Hélène, Tigaud Sylvestre, Laurent Frédéric
机构信息
National Reference Centre for Staphylococci, International Centre of Infectious Research, INSERM U1111, CNRS UMR5308, University of Lyon 1, ENS de Lyon, Lyon, France; Departments of Clinical Microbiology, Hospices Civils de Lyon, Lyon, France.
National Reference Centre for Staphylococci, International Centre of Infectious Research, INSERM U1111, CNRS UMR5308, University of Lyon 1, ENS de Lyon, Lyon, France.
出版信息
Diagn Microbiol Infect Dis. 2016 Nov;86(3):262-264. doi: 10.1016/j.diagmicrobio.2016.08.012. Epub 2016 Aug 13.
We evaluated the performance of an immunochromatographic assay (PBP2a Culture Colony Test - Alere™), detecting protein-binding penicillin 2a on staphylococci primary isolates in only 6minutes. The assay is highly sensitive for the direct detection of MRSA on various culture media whereas it requires cefoxitin induction for methicillin-resistant coagulase-negative staphylococci.
我们评估了一种免疫层析检测法（PBP2a培养菌落检测 - 雅培™）的性能，该方法仅需6分钟就能检测葡萄球菌原始分离株上的蛋白结合青霉素2a。该检测法对在各种培养基上直接检测耐甲氧西林金黄色葡萄球菌高度敏感，而对于耐甲氧西林凝固酶阴性葡萄球菌则需要头孢西丁诱导。
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