Khedir Sameh Ben, Bardaa Sana, Chabchoub Naourez, Moalla Dorsaf, Sahnoun Zouheir, Rebai Tarek
a Laboratory of Histology, Embryology and Reproductive Biology, Faculty of Medicine of Sfax , University of Sfax , Sfax, Tunisia.
b Laboratory of Pharmacology, Faculty of Medicine of Sfax , University of Sfax , Sfax, Tunisia.
Pharm Biol. 2017 Dec;55(1):1407-1414. doi: 10.1080/13880209.2016.1233569. Epub 2016 Sep 23.
Since antiquity, Pistacia lentiscus L. (Anacardiaceae) fruit oil (PLFO) has been used as a remedy for primary health care such as burn treatment.
This study assesses the healing effect of PLFO on CO laser fractional burn in a rat model.
The study was carried out on 18 adult male Wistar rats. A second-degree laser burn (wound area = 2.2 cm) was inflicted in the dorsal region by the application of CO fractional laser within the following parameters; Energy level: 25 MJ and Depth level: 4. After applying laser, the rats were divided into three groups: the first was treated with saline solution, the second with a reference cream 'CYTOL BASIC' (0.13 μg/mm) and the third with PLFO (0.52 μL/mm). All treatments were topically administered for eight days. The healing effect was assessed using macroscopic, histological and biochemical parameters.
After eight days, the higher percentage of wound healing contraction was observed among the PLFO-treated group (100%) followed by the 'CYTOL BASIC' treated group (61.36%) and untreated group (32.27%). During the treatment, the PLFO-treated group showed less erythema, less crusting/scabbing, higher general wound appearance scores and a high content of collagen (220.67 ± 7.48 mg/g of tissue) than the other groups.
The current study has shown, for the first time, the healing effect of PLFO on CO laser fractional burn. Their wound healing effect could be attributed to their antimicrobial, anti-inflammatory and antioxidant effects.
自古以来，乳香黄连木（漆树科）果油（PLFO）就被用作初级卫生保健的药物，如烧伤治疗。
本研究评估PLFO对大鼠模型中CO2激光分次烧伤的愈合效果。
对18只成年雄性Wistar大鼠进行研究。在背部区域应用CO2分次激光，在以下参数范围内造成二度激光烧伤（伤口面积 = 2.2 cm）；能量水平：25 mJ，深度水平：4。激光照射后，将大鼠分为三组：第一组用生理盐水治疗，第二组用参考乳膏“CYTOL BASIC”（0.13 μg/mm）治疗，第三组用PLFO（0.52 μL/mm）治疗。所有治疗均局部给药8天。使用宏观、组织学和生化参数评估愈合效果。
8天后，PLFO治疗组的伤口愈合收缩百分比最高（100%），其次是“CYTOL BASIC”治疗组（61.36%）和未治疗组（32.27%）。在治疗期间，PLFO治疗组比其他组表现出更少的红斑、更少的结痂/结疤、更高的总体伤口外观评分和高含量的胶原蛋白（220.67 ± 7.48 mg/g组织）。
本研究首次表明PLFO对CO2激光分次烧伤具有愈合效果。其伤口愈合效果可能归因于其抗菌、抗炎和抗氧化作用。
