Schöll Michael Johannes, Santos Edgar, Sanchez-Porras Renan, Kentar Modar, Gramer Markus, Silos Humberto, Zheng Zelong, Gang Yuan, Strong Anthony John, Graf Rudolf, Unterberg Andreas, Sakowitz Oliver W, Dickhaus Hartmut
1 Institute of Medical Biometry and Informatics, University Hospital Heidelberg, Heidelberg, Germany.
2 Department of Neurosurgery, University Hospital Heidelberg, Heidelberg, Germany.
J Cereb Blood Flow Metab. 2017 May;37(5):1706-1719. doi: 10.1177/0271678X16668988. Epub 2016 Jan 1.
Haemodynamic responses to spreading depolarizations (SDs) have an important role during the development of secondary brain damage. Characterization of the haemodynamic responses in larger brains, however, is difficult due to movement artefacts. Intrinsic optical signal (IOS) imaging, laser speckle flowmetry (LSF) and electrocorticography were performed in different configurations in three groups of in total 18 swine. SDs were elicited by topical application of KCl or occurred spontaneously after middle cerebral artery occlusion. Movement artefacts in IOS were compensated by an elastic registration algorithm during post-processing. Using movement-compensated IOS, we were able to differentiate between four components of optical changes, corresponding closely with haemodynamic variations measured by LSF. Compared with ECoG and LSF, our setup provides higher spatial and temporal resolution, as well as a better signal-to-noise ratio. Using IOS alone, we could identify the different zones of infarction in a large gyrencephalic middle cerebral artery occlusion pig model. We strongly suggest movement-compensated IOS for the investigation of the role of haemodynamic responses to SDs during the development of secondary brain damage and in particular to examine the effect of potential therapeutic interventions in gyrencephalic brains.
对扩散性去极化（SDs）的血流动力学反应在继发性脑损伤的发展过程中起着重要作用。然而，由于运动伪影，在较大的大脑中表征血流动力学反应很困难。在总共18头猪的三组中，以不同配置进行了内在光学信号（IOS）成像、激光散斑血流仪（LSF）和皮层脑电图检查。通过局部应用氯化钾诱发SDs，或在大脑中动脉闭塞后自发发生。在后期处理过程中，通过弹性配准算法补偿IOS中的运动伪影。使用运动补偿的IOS，我们能够区分光学变化的四个成分，这与LSF测量的血流动力学变化密切相关。与皮层脑电图和LSF相比，我们的设置提供了更高的空间和时间分辨率，以及更好的信噪比。仅使用IOS，我们就能在一个大脑回沟丰富的大脑中动脉闭塞猪模型中识别出不同的梗死区域。我们强烈建议使用运动补偿的IOS来研究血流动力学反应在继发性脑损伤发展过程中对SDs的作用，特别是研究潜在治疗干预对大脑回沟丰富的大脑的影响。
