Sun Yanping, Xian Lei, Yu Jiankun, Yang Tianzhi, Zhang Jinmin, Yang Zhen, Jiang Jingzheng, Cai Cuifang, Zhao Xiaoyun, Yang Li, Ding Pingtian
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Department of Basic Pharmaceutical Sciences, School of Pharmacy, Husson University, Bangor, ME 04401-2929, USA.
Macromol Biosci. 2017 Mar;17(3). doi: 10.1002/mabi.201600297. Epub 2016 Sep 29.
Poly(amido amine)s' (PAAs) versatility are nearly unique among stepwise polymers. Different functional groups can be easily introduced into these polymers to add functionality such as cell internalization, charge-shift, bioreducibility, "stealth" properties, and targeting moieties, while maintaining the bulk structural integrity of these polymers. The poly(amido amine)s are used as a unique research platform to elucidate their complex structure-function relationship. It is shown that guanidinium group, carboxyl group, disulfide bond, alkyl chain, branching, acetyl groups, benzoyl groups, and quaternary nicotinamide moieties can influence many steps of gene delivery, such as DNA condensation, cellular uptake, endosomal escape, nuclear entry, and finally gene expression. The authors systematically discuss the structure-function correlations of PAAs for gene delivery, and elaborate how the properties of polymers can be adjusted by changing the polymeric structure.
聚（酰胺胺）（PAA）的多功能性在逐步聚合的聚合物中几乎是独一无二的。不同的官能团可以很容易地引入到这些聚合物中，以增加诸如细胞内化、电荷转移、生物还原性、“隐身”特性和靶向部分等功能，同时保持这些聚合物的整体结构完整性。聚（酰胺胺）被用作一个独特的研究平台，以阐明其复杂的结构-功能关系。结果表明，胍基、羧基、二硫键、烷基链、支化、乙酰基、苯甲酰基和季铵化烟酰胺部分可以影响基因传递的许多步骤，如DNA凝聚、细胞摄取、内体逃逸、核进入，以及最终的基因表达。作者系统地讨论了用于基因传递的PAA的结构-功能相关性，并阐述了如何通过改变聚合物结构来调节聚合物的性能。
