Siu L Kristopher, Tsai Yu-Kuo, Lin Jung-Chung, Chen Te-Li, Fung Chang-Phone, Chang Feng-Yee
KeMyth Biotechnology Company, Incubation Center of Yuanpei University of Medical Technology, Hsinchu, Taiwan.
Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan.
J Clin Microbiol. 2016 Dec;54(12):3018-3021. doi: 10.1128/JCM.01608-16. Epub 2016 Oct 5.
In this study, a novel colloidal gold-based immunochromatographic strip (ICS) containing anti-Klebsiella pneumoniae capsular polysaccharide polyclonal antibodies was developed to specifically detect K. pneumoniae serotypes K1 and K2. Capsular polysaccharide K1 and K2 antigens were first used to produce polyclonal anti-K1 and anti-K2 antibodies. Reference strains with different serotypes, nontypeable K. pneumoniae strains, and other bacterial species were then used to assess the sensitivity and specificity of these test strips. The detection limit was found to be 10 CFU, and the ICSs were stable for 6 months when stored at room temperature. No false-positive or false-negative results were observed, and equivalent results were obtained compared to those of more conventional test methods, such as PCR or serum agglutination. In conclusion, the ICS developed here requires no technical expertise and allows for the specific, rapid, and simultaneous detection of K. pneumoniae serotypes K1 and K2.
在本研究中，开发了一种新型的基于胶体金的免疫层析试纸条（ICS），其含有抗肺炎克雷伯菌荚膜多糖多克隆抗体，用于特异性检测肺炎克雷伯菌血清型K1和K2。首先使用荚膜多糖K1和K2抗原制备多克隆抗K1和抗K2抗体。然后使用不同血清型的参考菌株、不可分型的肺炎克雷伯菌菌株以及其他细菌物种来评估这些试纸条的敏感性和特异性。检测限为10 CFU，当在室温下储存时，ICS可稳定保存6个月。未观察到假阳性或假阴性结果，并且与更传统的检测方法（如PCR或血清凝集）相比，获得了等效的结果。总之，此处开发的ICS不需要技术专长，并且能够特异性、快速且同时检测肺炎克雷伯菌血清型K1和K2。
