Verigene®肠道病原体检测、Biofire FilmArray™胃肠道检测板及Luminex xTAG®胃肠道病原体检测板用于检测常见肠道病原体的性能

Performance of the Verigene® enteric pathogens test, Biofire FilmArray™ gastrointestinal panel and Luminex xTAG® gastrointestinal pathogen panel for detection of common enteric pathogens.

作者信息

Huang Richard S P, Johnson Coreen L, Pritchard Lauryn, Hepler Richard, Ton Trang T, Dunn James J

机构信息

Dept. of Pathology, Texas Children's Hospital, Houston, TX, USA; Dept. of Pathology & Immunology, Baylor College of Medicine, Houston, TX, USA.

Dept. of Pathology, Texas Children's Hospital, Houston, TX, USA.

出版信息

Diagn Microbiol Infect Dis. 2016 Dec;86(4):336-339. doi: 10.1016/j.diagmicrobio.2016.09.013. Epub 2016 Sep 22.

DOI:10.1016/j.diagmicrobio.2016.09.013

PMID:27720206

Abstract

BACKGROUND

Multiplex syndromic panels have the capability of identifying causes of diarrheal illness. This study evaluated the performance characteristics of three multiplex molecular assays for the detection of common stool pathogens.

METHODS

A total of 152 stool specimens were tested using three platforms: Verigene Enteric Pathogens Test (Verigene), Biofire FilmArray Gastrointestinal Panel (Biofire) and Luminex xTAG® Gastrointestinal Pathogen Panel (Luminex). Assays were assessed only for the targets common among all three; namely, Campylobacter, Salmonella, Shigella, Shiga toxin-producing E. coli (STEC), norovirus, and rotavirus.

RESULTS

The sensitivities (%) and specificities (%) of the assays were: Campylobacter, Biofire (100,100), Verigene (83.3,99.3), Luminex (91.7,100); Salmonella, Biofire (95.8,100), Verigene (83.3,100), Luminex (79.2,100); Shigella, Biofire (100,100), Verigene (95.4,99.1), Luminex (100,100); STEC, Biofire (100,100), Verigene (91.7,100), Luminex (91.7,100); norovirus, Biofire (94.7,99.3), Verigene (89.0,100), Luminex (89.5,100); and rotavirus, Biofire (100, 98.6), Verigene (71.4,100), Luminex (100,100).

CONCLUSIONS

All multiplex panels detected the majority of gastrointestinal pathogens when compared to conventional methods.

摘要

背景

多重症状检测板有能力识别腹泻病的病因。本研究评估了三种多重分子检测方法用于检测常见粪便病原体的性能特征。

方法

使用三种平台对总共152份粪便标本进行检测：Verigene肠道病原体检测（Verigene）、Biofire FilmArray胃肠道检测板（Biofire）和Luminex xTAG®胃肠道病原体检测板（Luminex）。仅针对所有三种检测方法共有的目标进行评估，即弯曲杆菌、沙门氏菌、志贺氏菌、产志贺毒素大肠杆菌（STEC）、诺如病毒和轮状病毒。

结果

各检测方法的敏感性（%）和特异性（%）如下：弯曲杆菌，Biofire（100,100），Verigene（83.3,99.3），Luminex（91.7,100）；沙门氏菌，Biofire（95.8,100），Verigene（83.3,100），Luminex（79.2,100）；志贺氏菌，Biofire（100,100），Verigene（95.4,99.1），Luminex（100,100）；STEC，Biofire（100,100），Verigene（91.7,100），Luminex（91.7,100）；诺如病毒，Biofire（94.7,99.3），Verigene（89.0,100），Luminex（89.5,100）；轮状病毒，Biofire（100, 98.6），Verigene（71.4,100），Luminex（100,100）。