超分辨率显微镜揭示高尔基体后囊泡上转化生长因子受体的纳米级分布
Nanoscale Distribution of Transforming Growth Factor Receptor on Post-Golgi Vesicle Revealed by Super-resolution Microscopy.
作者信息
Ruan Hefei, Yu Jianqiang, Yuan Jinghe, Li Nan, Fang Xiaohong
机构信息
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Nanostructures and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences, 2 ZhongGuanCun North First Street, Beijing, 100190, P.R. China), Fax: (+86) 10-62653083.
University of Chinese Academy of Sciences, Beijing, 100049, P.R. China.
出版信息
Chem Asian J. 2016 Dec 6;11(23):3359-3364. doi: 10.1002/asia.201600436. Epub 2016 Nov 3.
Transforming growth factor-β (TGF-β) type II receptor (TβRII) plays a critical role in the initiation of TGF-β signaling pathway; therefore, the study of its synthesis and transport processes is of great important. In this work, we achieved super-resolution imaging of a new type of TβRII-containing post-Golgi vesicle by our home-built stimulated emission depletion (STED) microscope. We visualized the ring-shaped structure of these vesicles containing newly synthesized TβRII in the cytoplasm and characterized their size distribution from 300 to 1000 nm. These vesicles could be swollen by chloroquine treatment. Further investigation revealed that TβRII formed clusters on the outer ring of the post-Golgi vesicles. This study offers new information on the intracellular transportation of TGF-β receptors for better understanding its signaling process.
转化生长因子-β（TGF-β）Ⅱ型受体（TβRII）在TGF-β信号通路的启动中起关键作用；因此，对其合成和运输过程的研究非常重要。在这项工作中，我们通过自制的受激发射损耗（STED）显微镜实现了对一种新型含TβRII的高尔基体后囊泡的超分辨率成像。我们观察到这些在细胞质中含有新合成TβRII的囊泡的环形结构，并对其300至1000纳米的大小分布进行了表征。这些囊泡可通过氯喹处理而肿胀。进一步研究表明，TβRII在高尔基体后囊泡的外环上形成簇。这项研究为TGF-β受体的细胞内运输提供了新信息，以更好地理解其信号传导过程。
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