微粒体谷胱甘肽依赖性抗脂质过氧化作用是通过大鼠肝脏中除谷胱甘肽过氧化物酶和谷胱甘肽S-转移酶之外的一种因子发挥的。

Microsomal glutathione-dependent protection against lipid peroxidation acts through a factor other than glutathione peroxidase and glutathione S-transferase in rat liver.

作者信息

Nagasaka Y, Fujii S, Kaneko T

机构信息

Third Department of Internal Medicine, Yamaguchi University, School of Medicine, Japan.

出版信息

Arch Biochem Biophys. 1989 Oct;274(1):82-6. doi: 10.1016/0003-9861(89)90417-7.

DOI:10.1016/0003-9861(89)90417-7

PMID:2774585

Abstract

Ascorbate-Fe3+-induced and NADPH-induced lipid peroxidation of rat liver microsomes were inhibited by glutathione (GSH). This inhibition was due to microsomal GSH-dependent factor. This factor was heat labile, and storage of microsomes at 4 degrees C for 1 week diminished the activity. GSH could not be substituted by other sulfhydryl compounds tested. Deoxycholate (1 mM) and bromosulfophthalein (0.1 mM) inhibited GSH-dependent protection but did not inhibit microsomal GSH peroxidase activity. Iodoacetate (10 mM) inhibited GSH-dependent protection but did not inhibit microsomal GSH S-transferase. N-Ethylmaleimide (0.1 mM) and oxidized glutathione (10 mM) inhibited GSH-dependent protection but activated microsomal GSH S-transferase activity. These results indicate the existence of a heat-labile, microsomal GSH-dependent protective factor against lipid peroxidation that acts through a factor other than GSH-peroxidase and GSH S-transferase.

摘要

谷胱甘肽（GSH）可抑制抗坏血酸盐 - 铁离子诱导的以及NADPH诱导的大鼠肝微粒体脂质过氧化。这种抑制作用归因于微粒体中依赖GSH的因子。该因子对热不稳定，微粒体在4℃储存1周会使其活性降低。所测试的其他巯基化合物不能替代GSH。脱氧胆酸盐（1 mM）和溴磺酞（0.1 mM）可抑制依赖GSH的保护作用，但不抑制微粒体GSH过氧化物酶活性。碘乙酸盐（10 mM）抑制依赖GSH的保护作用，但不抑制微粒体GSH S - 转移酶。N - 乙基马来酰亚胺（0.1 mM）和氧化型谷胱甘肽（10 mM）抑制依赖GSH的保护作用，但激活微粒体GSH S - 转移酶活性。这些结果表明存在一种对脂质过氧化具有保护作用的、热不稳定的、微粒体中依赖GSH的因子，其作用途径不同于GSH过氧化物酶和GSH S - 转移酶。