Isac Eliana, de A Picanço Guaraciara, da Costa Tatiane L, de Lima Nayana F, de S M M Alves Daniella, Fraga Carolina M, de S Lino Junior Ruy, Vinaud Marina C
Laboratory of Studies of the Host-Parasite Relationship, Tropical Pathology and Public Health Institute, Federal University of Goias, Rua 235, s/n, Setor Universitário, Goiania, Goias, CEP:74605-050, Brazil.
Laboratory of Studies of the Host-Parasite Relationship, Tropical Pathology and Public Health Institute, Federal University of Goias, Rua 235, s/n, Setor Universitário, Goiania, Goias, CEP:74605-050, Brazil.
Exp Parasitol. 2016 Dec;171:17-22. doi: 10.1016/j.exppara.2016.10.012. Epub 2016 Oct 14.
Nitazoxanide (NTZ) is a broad-spectrum anti-parasitic drug used against a wide variety of protozoans and helminthes. Albendazole, its active metabolite albendazole sulfoxide (ABZSO), is one of the drugs of choice to treat both intestinal and tissue helminth and protozoan infections. However little is known regarding their impact on the metabolism of parasites. The aim of this study was to compare the in vitro effect of NTZ and ABZSO in the glycolysis of Taenia crassiceps cysticerci. The cysticerci were treated with 1.2; 0.6; 0.3 or 0.15 μg/mL of NTZ or ABZSO. Chromatographic and spectrophotometric analyses were performed in the culture medium and in the cysticerci extract. Regarding the glucose concentrations was possible to observe two responses: impair of the uptake and gluconeogenesis. The pyruvate concentrations were increased in the ABZSO treated group. Lactate concentrations were increased in the culture medium of NTZ treated groups. Therefore it was possible to infer that the metabolic acidosis was greater in the group treated with NTZ than in the ABZSO treated group indicating that this is one of the modes of action used by this drug to induce the parasite death.
硝唑尼特（NTZ）是一种广谱抗寄生虫药物，可用于对抗多种原生动物和蠕虫。阿苯达唑及其活性代谢产物阿苯达唑亚砜（ABZSO）是治疗肠道和组织蠕虫及原生动物感染的首选药物之一。然而，关于它们对寄生虫代谢的影响却知之甚少。本研究的目的是比较硝唑尼特和阿苯达唑亚砜在粗颈带绦虫囊尾蚴糖酵解过程中的体外作用。用1.2、0.6、0.3或0.15μg/mL的硝唑尼特或阿苯达唑亚砜处理囊尾蚴。在培养基和囊尾蚴提取物中进行色谱和分光光度分析。关于葡萄糖浓度，可以观察到两种反应：摄取和糖异生受损。阿苯达唑亚砜处理组的丙酮酸浓度升高。硝唑尼特处理组培养基中的乳酸浓度升高。因此，可以推断，硝唑尼特处理组的代谢性酸中毒比阿苯达唑亚砜处理组更严重，这表明这是该药物诱导寄生虫死亡的作用方式之一。
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