Meng Xiaodan, Müller Volkmar, Milde-Langosch Karin, Trillsch Fabian, Pantel Klaus, Schwarzenbach Heidi
Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, Hamburg-Eppendorf, 20246, Germany.
Department of Gynecology, University Medical Center Hamburg-Eppendorf, Hamburg-Eppendorf, 20246, Germany.
Adv Exp Med Biol. 2016;924:3-8. doi: 10.1007/978-3-319-42044-8_1.
In the present study, we investigated whether circulating cell-free microRNAs serve as potential biomarkers in epithelial ovarian cancer (EOC) patients. Circulating miR-373, miR-200a, miR-200b and miR-200c were quantified in a cohort of 60 EOC patients, 20 patients with benign ovarian diseases and 32 healthy women using quantitative TaqMan MicroRNA assays. The serum concentrations of cell-free miR-373, miR-200a, miR-200b and miR-200c were significantly higher in EOC patients than in healthy women (p = 0.0001). With a sensitivity of 83 % and a specificity of 100 %, the combination of miR-200a, miR-200b and miR-200c could differ between malignant and benign ovarian tumors (p = 0.0001). Elevated levels of these cell-free microRNAs could be detected in FIGO I-II and FIGO III-IV stages, grading G1-2 and G3 and lymph node-negative and -positive EOC. In conclusion, the increased serum levels of this microRNA panel have diagnostic value for distinguishing healthy controls and benign tumors from EOC.
在本研究中，我们调查了循环游离微小RNA是否可作为上皮性卵巢癌（EOC）患者的潜在生物标志物。使用定量TaqMan微小RNA检测法，对60例EOC患者、20例良性卵巢疾病患者和32名健康女性进行了循环miR-373、miR-200a、miR-200b和miR-200c的定量分析。EOC患者血清中游离miR-373、miR-200a、miR-200b和miR-200c的浓度显著高于健康女性（p = 0.0001）。miR-200a、miR-200b和miR-200c联合检测对恶性和良性卵巢肿瘤的鉴别诊断具有83%的敏感性和100%的特异性（p = 0.0001）。在国际妇产科联盟（FIGO）I-II期和FIGO III-IV期、G1-2级和G3级以及淋巴结阴性和阳性的EOC患者中均检测到这些游离微小RNA水平升高。总之，该微小RNA组合血清水平升高对区分健康对照、良性肿瘤与EOC具有诊断价值。
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