Güçlü Aydın, Koçak Cengiz, Koçak Fatma Emel, Akçılar Raziye, Dodurga Yavuz, Akçılar Aydın, Seçme Mücahit
a Department of Nephrology , Ahi Evran School of Medicine , Kırşehir , Turkey.
b Department of Pathology , Dumlupınar School of Medicine , Kütahya , Turkey.
Ren Fail. 2016 Oct;38(9):1468-1475. doi: 10.1080/0886022X.2016.1227915. Epub 2016 Oct 19.
MicroRNAs (miR) are important diagnostic and treatment targets due to their different tissue expressions and their central position in the regulation of gene expressions. miR studies might pioneer emerging of new diagnostic tools and treatment goals in kidney diseases. Captopril (CAP) and telmisartan (TEL) were shown to be effective in ischemia reperfusion (IR) injury. There is not any study about the effect of TEL and CAP over miR-21-320-146a. Our aim was to study the effects of CAP and TEL over miR on renal IR model.
We used 12-16 weeks-old Wistar-Albino rats that weigh 300-350 g. Rats (n, 6) were randomized into four groups (Control, IR, IR + CAP, IR + TEL). Urea, creatinine, total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), super oxide dismutase (SOD), and miRs were analyzed.
Urea, creatinine, TOS, OSI levels of IR + CAP, and IR + TEL groups were lower comparing to IR group. TAS and SOD levels were higher in IR group than IR + TEL group. miR-21-320-146a showed increase in renal IR injury. miR-320, 146a showed significant decrease in IR + CAP and IR + TEL groups comparing to IR group. We showed histopathological recovery and decreased apoptosis in IR + CAP and IR + T groups than IR group.
We, for the first time in the literature, showed that miR-320 is increased in IR injury. miR-320 might be a novel diagnosis and treatment target in renal ischemic reperfusion injury. Also, for the first time, we showed that CAP and TEL cause functional and histopathological recovery and lower miR-146a and miR-320.
微小RNA（miR）因其在不同组织中的表达差异以及在基因表达调控中的核心地位，成为重要的诊断和治疗靶点。miR研究可能引领肾脏疾病新诊断工具和治疗目标的出现。卡托普利（CAP）和替米沙坦（TEL）已被证明对缺血再灌注（IR）损伤有效。关于TEL和CAP对miR-21-320-146a的影响尚无研究。我们的目的是研究CAP和TEL对肾脏IR模型中miR的影响。
我们使用12至16周龄、体重300至350克的Wistar-白化大鼠。大鼠（n = 6）被随机分为四组（对照组、IR组、IR + CAP组、IR + TEL组）。分析尿素、肌酐、总抗氧化状态（TAS）、总氧化状态（TOS）、氧化应激指数（OSI）、超氧化物歧化酶（SOD）和miR。
与IR组相比，IR + CAP组和IR + TEL组的尿素、肌酐、TOS、OSI水平较低。IR组的TAS和SOD水平高于IR + TEL组。miR-21-320-146a在肾脏IR损伤中呈升高趋势。与IR组相比，IR + CAP组和IR + TEL组中的miR-320、146a显著降低。我们发现IR + CAP组和IR + T组相较于IR组，组织病理学恢复且凋亡减少。
我们首次在文献中表明，miR-320在IR损伤中升高。miR-320可能是肾脏缺血再灌注损伤的新型诊断和治疗靶点。此外，我们首次表明CAP和TEL可导致功能和组织病理学恢复，并降低miR-146a和miR-320。
