Başpınar Emel Ödemiş, Dayan Saim, Bekçibaşı Muhammed, Tekin Recep, Ayaz Celal, Deveci Özcan, Hoşoğlu Salih
Celal Ertuğ Etimesgut State Hospital, Department of Infectious Diseases and Clinical Microbiology, Ankara, Turkey.
Dicle University School of Medicine, Department of Infectious Diseases and Clinical Microbiology, Diyarbakır, Turkey.
Braz J Microbiol. 2017 Apr-Jun;48(2):232-236. doi: 10.1016/j.bjm.2016.06.014. Epub 2016 Oct 6.
Our aim in this study is to compare the standard culture method with the multiplex PCR and the Speed-Oligo Bacterial Meningitis Test (SO-BMT) - a hybridization-based molecular test method - during the CSF examination of the patients with the pre-diagnosis of acute bacterial meningitis. For the purposes of this study, patients with acute bacterial meningitis treated at the Dicle University Medical Faculty Hospital, Infectious Diseases and Clinical Microbiology Clinic between December 2009 and April 2012 were retrospectively evaluated. The diagnosis of bacterial meningitis was made based on the clinical findings, laboratory test anomalies, CSF analysis results, and the radiological images. Growth was observed in the CSF cultures of 10 out of the 57 patients included in the study (17.5%) and Streptococcus pneumoniae was isolated in all of them. The CSF samples of 34 patients (59.6%) were positive according to the SO-BMT and S. pneumoniae was detected in 33 of the samples (97.05%), while Neisseria meningitidis was found in 1 sample (2.95%). In a total of 10 patients, S. pneumoniae was both isolated in the CSF culture and detected in the SO-BMT. The culture and the SO-BMT were negative in 23 of the CSF samples. There was no sample in which the CSF culture was positive although the SO-BMT was negative. While SO-BMT seems to be a more efficient method than bacterial culturing to determine the pathogens that most commonly cause bacterial meningitis in adults, further studies conducted on larger populations are needed in order to assess its efficiency and uses.
本研究的目的是在对预先诊断为急性细菌性脑膜炎的患者进行脑脊液检查时，将标准培养方法与多重聚合酶链反应（PCR）以及速寡核苷酸细菌性脑膜炎检测法（SO - BMT，一种基于杂交的分子检测方法）进行比较。为了本研究的目的，对2009年12月至2012年4月期间在狄克莱大学医学院医院传染病与临床微生物学诊所接受治疗的急性细菌性脑膜炎患者进行了回顾性评估。细菌性脑膜炎的诊断基于临床症状、实验室检查异常、脑脊液分析结果以及影像学图像。在纳入研究的57例患者中，有10例（17.5%）的脑脊液培养有细菌生长，且所有病例均分离出肺炎链球菌。根据SO - BMT检测，34例患者（59.6%）的脑脊液样本呈阳性，其中33例样本（97.05%）检测出肺炎链球菌，1例样本（2.95%）检测出脑膜炎奈瑟菌。共有10例患者的脑脊液培养中分离出肺炎链球菌，且在SO - BMT检测中也检测到该菌。23例脑脊液样本的培养和SO - BMT检测均为阴性。没有出现SO - BMT检测为阴性而脑脊液培养为阳性的样本。虽然在确定成人细菌性脑膜炎最常见的病原体方面，SO - BMT似乎比细菌培养更有效，但为了评估其效率和用途，还需要对更多人群进行进一步研究。
